Specific Effect of Polyunsaturated Fatty Acids on the Cholesterol-Poor Membrane Domain in a Model Membrane
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概要
- 論文の詳細を見る
To understand more fully the effect of polyunsaturated fatty acids (PUFAs) on lipid bilayers, we investigated the effects of treatment with fatty acids on the properties of a model membrane. Three kinds of liposomes comprising dipalmitoylphosphatidylcholine (DPPC), dioleylphosphatidylcholine (DOPC), and cholesterol (Ch) were used as the model membrane, and the fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) and detergent insolubility were determined. Characterization of the liposomes clarified that DPPC, DPPC/Ch, and DPPC/DOPC/Ch existed as solid-ordered phase (L_β), liquid-ordered phase (l_o), and a mixture of l_o and liquid-disordered phase (L_α) membranes at room temperature. Treatment with unsaturated fatty acids such as oleic acid (OA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) markedly decreased the fluorescence anisotropy value and detergent insolubility. PUFAs and OA had different effects on the model membranes. In DPPC liposomes, the most prominent change was induced by PUFAs, whereas, in DPPC/Ch and DPPC/DOPC/Ch liposomes, OA had a stronger effect than PUFAs. The effect of PUFAs was strongly affected by the amount of Ch in the membrane, which confirmed a specific effect of PUFAs on the Ch-poor membrane domain. We further explored the effect of fatty acids dispersed in a water-in-oil-in-water multiple emulsion and found that unsaturated fatty acids acted on the membranes even when incorporated in emulsion form. These findings suggest that treatment with PUFAs increases the segregation of ordered and disordered phase domains in membranes.
- 公益社団法人日本薬学会の論文
- 2008-08-01
著者
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Takayama Kozo
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Hoshi University
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Onuki Yoshinori
Department Of Pharmaceutics Hoshi University
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HAGIWARA Chihiro
Department of Pharmaceutics, Hoshi University
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SUGIBAYASHI Ko
Department of Pharmaceutics, Hoshi University
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Hagiwara Chihiro
Department Of Pharmaceutics Hoshi University
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Sugibayashi Ko
Department Of Pharmaceutics Hoshi University
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Takayama Kozo
Department of Drug Delivery Research, Hoshi University
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