Spin-Trapping Studies on the Reaction of Iron Complexes with Peroxides and the Effects of Water-Soluble Antioxidants.
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概要
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Using ESR spin-trapping techniques we measured the levels of free radical species generated from six different systems (hypoxanthine-xanthine oxidase, iron(II)–hydrogen peroxide, iron(III)–hydrogen peroxide, iron(II)–<I>t</I>-butyl hydroperoxide, iron(III)–<I>t</I>-butyl hydroperoxide, and catalase (CAT)–<I>t</I>-butyl hydroperoxide). Six types of radicals (O<SUB>2</SUB><SUP>−·</SUP>,·OH, ·H, ·CH<SUB>3</SUB>, (CH<SUB>3</SUB>)<SUB>3</SUB>CO·, and (CH<SUB>3</SUB>)<SUB>3</SUB>COO·) were detected as spin adducts of spin traps 5,5-dimethylpyrroline 1-oxide (DMPO) or 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS). Quantitative analysis of the levels of generated radicals by means of an ESR instrument also presents important information regarding the reduction of peroxides [hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) or <I>t</I>-butyl hydroperoxide (ROOH)] by iron(II) or iron(III) as well as catalase. In addition, the scavenging potencies of different water-soluble antioxidants such as L-ascorbic, D-isoascorbic, gallic, sorbic, and protocatechuic acids were evaluated in terms of their ability to reduce the peaks of spin adducts.
- 公益社団法人 日本化学会の論文
著者
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Yoshikawa Toshikazu
First Department Internal Medicine Kyoto Prefectural University Of Medicine
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Kohno Masahiro
Esr Application Laboratory Application And Research Center Analytical Instruments Division Jeol Ltd.
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Mitsuta Keiichi
Esr Application Laboratory Application And Research Center Analytical Instruments Division Jeol Ltd.
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YOSHIKAWA Toshikazu
First Department of Medicine, Kyoto Prefectural University of Medicine
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Kohno Masahiro
ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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Mitsuta Keiichi
ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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Yamada Masako
ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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Mizuta Yokio
ESR Application Laboratory, Analytical Instruments Division, JEOL Ltd.
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