The application of ESR spin-trapping technique to the evaluation of SOD-like activity of biological substances.
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概要
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The SOD-<I>like</I> activity of several biological substances was evaluated by an ESR spin-trapping technique. Superoxide radicals (O<SUB>2</SUB><SUP>\newdot</SUP>) were supplied enzymatically from a hypoxanthine–xanthine oxidase reaction to the evaluating system. By using a spin trap 5,5-dimethyl-1-pyrroline <I>N</I>-oxide (DMPO), the generated O<SUB>2</SUB><SUP>\newdot</SUP> was trapped stoichiometrically (1:1) as the spin adduct of O<SUB>2</SUB><SUP>\newdot</SUP> (DMPO–O<SUB>2</SUB><SUP>−</SUP>). When biological substances were added to the system, a decrease in the ESR signal intensities of the adducts was observed. This phenomenon could be explained as being an inhibition of adduct formation, and related to the reactivity of added biological substances with O<SUB>2</SUB><SUP>\newdot</SUP>, called an SOD-<I>like</I> activity. By the method of kinetic competition with a 50% inhibitory dose (ID<SUB>50</SUB>), the second-order rate constant for the reaction between O<SUB>2</SUB><SUP>\newdot</SUP> and biological substance was determined. These rate constants can be used as a measure of the reactivity.
- 公益社団法人 日本化学会の論文
著者
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Mori Akitane
Department Of Neurochemistry Institute For Neurobiology Okayama University Medical School
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Mizuta Yukio
Esr Application Laboratory Jeol Ltd.
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Kohno Masahiro
Esr Application Laboratory Application And Research Center Analytical Instruments Division Jeol Ltd.
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Mitsuta Keiichi
Esr Application Laboratory Application And Research Center Analytical Instruments Division Jeol Ltd.
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Kohno Masahiro
ESR Application Laboratory, Application and Research Center, Analytical Instruments Division, JEOL Ltd.
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Hiramatsu Midori
Department of Neurochemistry, Institute for Neurobiology, Okayama University Medical School
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MORI Akitane
Department of Neurochemistry, Institute for Neurobiology, Okayama University Medical School
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