N-Linked Oligosaccharides of Glycoproteins from Ginkgo biloba Pollen, An Allergenic Pollen(Biochemistry & Molecular Biology)

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The pollen of Ginkgo biloba is one of the allergens that cause pollen allergy symptoms. The plant complex type N-glycans bearing β1-2 xylose and/or α1-3 fucose residue (s) linked to glycoallergens have been considered to be critical epitopes in various immune reactions. In this report, the structures of N-glycans of total glycoproteins prepared from Ginkgo biloba pollens were analyzed to confirm whether such plant complex type N-glycans occur in the pollen glycoproteins. The glycoproteins were extracted by SDS-Tris buffer. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine and the resulting pyridylaminated (PA-) N-glycans were purified by a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, IS-MS, and MS/MS. The plant complex type structures (GlcNAc_2Man_3Xyl_1 Fuc_1GlcNAc_2 (31%), GlcNAc_2Man_3Xyl_1GlcNAc_2 (5%), Man_3Xyl_1Fuc_1GlcNAc_2 (13%), GlcNAc_1Man_3 Xyl_1Fuc_1GlcNAc_2 (8%), and GlcNAc_1Man_3Xyl_1 GlcNAc_2 (17%)) have been found among the N-glycans of the glycoproteins of Ginkgo biloba pollen, which might be candidates for the epitopes involved in Ginkgo pollen allergy. The remaining 26% of the total pollen N-glycans have the typical high-mannose type structures : Man_8GlcNAc_2 (11%) and Man_6GlcNAc_2 (15%).
社団法人日本農芸化学会の論文
2001-09-23