Characterization and Affinity Purification of Juvenile Hormone Esterase from Bombyx mori

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概要

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• Juvenile hormone esterase (JHE) from hemolymph of the silkworm moth Bombyx mori was characterized for substrate specificity and inhibitor sensitivity. B. mori JHE hydrolyzed the juvenile hormone surrogate substrate methyl n-heptylthioacetothioate (HEPTAT) more efficiently than p-nitrophenyl acetate and 1-naphthyl acetate substrates widely used to assay total carboxylesterase activity. B. mori JHE was sensitive to 3-octylthio-1,1,1-trifluoro-2-propanone (OTFP), which was developed as a selective inhibitor for lepidopteran JHE, and relatively insensitive to diisopropyl fluorophosphate (DFP), an inhibitor of serine esterases but not of all JHEs. Affinity purification with a trifluoromethyl ketone ligand was more efficient for purification of B. mori JHE than DEAE ion exchange chromatography.

• 社団法人日本農芸化学会の論文
• 2000-08-23

著者

• Hirai Makoto

  Department Of Circulation And Respiration Research Institute Of Environmental Medicine Nagoya Univer

• KIKUCHI Kyoko

  Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences

• Kikuchi Kyoko

  Development And Differentiation Department National Institute Of Agrobiological Sciences

• HIRAI Makoto

  Development and Differentiation Department, National Institute of Agrobiological Sciences

• SHIOTSUKI Takahiro

  Department of Insect Physiology and Behavior, National Institute of Sericultural and Entomological S

• BONNING BryonyC.

  Department of Entomology, Iowa State University

• HAMMOCK BruceD.

  Department of Entomology, University of California

• Kikuchi Kyoko

  Department Of International And Cultural Studies Tsuda College

• Hammock Bruced.

  Department Of Entomology University Of California

• Bonning Bryonyc.

  Department Of Entomology Iowa State University

• Hirai Makoto

  Development And Differentiation Department National Institute Of Agrobiological Sciences

• Kikuchi Kaoru

  Development And Differentiation Department National Institute Of Agrobiological Sciences

• Shiotsuki T

  National Inst. Of Agrobiological Sci. Ibaraki Jpn

• Shiotsuki Takahiro

  Laboratory Of Insect Growth Regulation Developmental Biology Department National Institute Of Agrobi

• Shiotsuki Takahiro

  Department Of Insect Physiology And Behavior National Institute Of Sericultural And Entomological Sc

• Bonning Bryony

  Department of Entomology, Iowa State University

• Hirai Makoto

  Department of Cardiology, Nagoya University Graduate School of Health Science

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