Production of Extracellular NAD and NADP by a Lignin-Degrading Fungus, Phanerochaete chrysosporium : Metanolism of Pyridine Coenzymes in a Lignin-Degrading Fungus, Phanerochaete chrysosporium (II)

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A lignin-degrading fungus, Phanerochaete chrysosporium, was found to produce extracellular pyridine coenzymes. One gram of mycelium (based on dried mycelium) synthesized 2.52 μmol of NAD and NADH and 62% of the total amount was found in the culture medium. This fungus also produced 0.60 μmol of NADP and NADPH per gram of mycelium and 55% of these coenzymes was detected in the medium. Tracer experiments using radioactive nicotinic acid as a precursor also showed that NAD and NADP were excreted into the medium in a young culture. However, when the culture was continued, the degradation products of NAD, such as NMN and NAmRDPR, appeared in the medium. A nicotinic acid auxotroph of this fungus failed to grow in quinolinic acid-supplemented medium. Presence of quinolinate phosporibosyl transferase activity in the prototroph suggested that NAD in also produced via the de novo synthetic pathway.
社団法人日本生物工学会の論文
1984-06-25