Purification and Some Properties of Lignostilbene-α, β-dioxygenase Isozyme IV from Pseudomonas paucimobilis TMY1009

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概要

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• Lignostilbene-α, β-dioxygenase isozyme IV was purified from ultrasonic extracts of Pseudomonas paucimobilis TMY1009 through four steps of column chromatography. The fraction obtained gave a single band on SDS-PAGE and a single peak on reversed-phase HPLC and DEAE-HPLC. The specific activity and K_m of purified isozyme IV were 110μkat/g and 4.2μM for 4, 4'-dihydroxy-3, 3'-dimethoxystilbene, 150μkat/g and 3.3μM for 4, 2'-dihydroxy-3, 3'-dimethoxy-5'-(2"-carboxyvinyl)stilbene. The molecular mass of intact isozyme IV was estimated to be 94 kDa by gel permeation chromatography, and that of its subunits was 52 kDa by SDS-PAGE under denaturing conditions. The N-terminal amino acid sequence of isozyme IV differed slightly from that of other isozymes. Isozyme IV seemed to be composed of two identical subunits, γγ.

• 社団法人日本農芸化学会の論文
• 1997-09-23

著者

• SABURI Yoshimasa

  Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, University of

• KAMODA Shigehiro

  Department of Forest Products, Faculty of Agriculture, The University of Tokyo

• TERADA Tamami

  Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The Universit

• Saburi Yoshimasa

  Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences University Of T

• Kamoda Shigehiro

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

関連論文

• Inhibition of Trichoderma cellulase activity by a stilbene glucoside from Picea glehnii bark
• Purification and Some Properties of Lignostilbene-α,β-dioxygenase Responsible for the C_α-C_β Cleavage of a Diarylpropane Type Lignin Model Compound from Pseudomonas sp. TMY1009(Biological Chemistry)
• Purification and Some Properties of Two C_α-dehydrogenases Oxidizing Dimeric Lignin Model Compounds from Pseudomonas sp. TMY1009(Biological Chemistry)
• Production of Heterogeneous Dimer Lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli Cells
• A Common Structure of Substrate Shared by Lignostilbenedioxygenase Isozymes from Sphingomonas paucimobilis TMY1009(Microbiology & Fermentation Technology)
• Purification and Some Properties of Lignostilbene-α, β-dioxygenase Isozyme IV from Pseudomonas paucimobilis TMY1009
• Cloning, Expression, and Sequence Analysis of a Lignostilbene-α, β-dioxygenase Gene from Pseudomonas paucimobilis TMY1009
• Cloning of a Lignostilbene-α,β-dioxy-genase Gene from Pseudomonas paucimobilis TMY1009(Microbiology & Fermentation Industry)
• Structural and Enzymatical Comparison of Lignostilbene-α,β-dioxygenase Isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009
• Cloning of a Lignostilbene-α,β-dioxygenase Isozyme Gene from Pseudomonas Paucimobilis TMY1009

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