Structural and Enzymatical Comparison of Lignostilbene-α,β-dioxygenase Isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009
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概要
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Three isozymes of lignostilbene-α, β-dioxygenase (LSD) from Pseudomonas paucimobilis TMY1009 were separated on QAE-Toyopearl chromatography. All active fractions were further chromatographed on DEAE-Toyopearl, Butyl-Toyopearl, and Sephacryl S-300 columns. Then the isozymes I, II, and III were purified homogeneously. All three isozymes consisted of two subunits with the same mol. mass. According to the N-terminal amino acid sequences up to 25 residues of these three isozymes and the reversed-phase HPLC patterns of peptidase-digested them, it was found that LSD-I, II, and III consisted of αα, αβ, and ββ subunits, respectively. They showed different specificities for several substrates that are stilbene and styrene derivatives.
- 社団法人日本農芸化学会の論文
- 1993-06-23
著者
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KAMODA Shigehiro
Department of Forest Products, Faculty of Agriculture, The University of Tokyo
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Saburi Yoshimasa
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
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Saburi Yoshimasa
Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences University Of T
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Kamoda Shigehiro
Department Of Forest Products Faculty Of Agriculture The University Of Tokyo
関連論文
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- Production of Heterogeneous Dimer Lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli Cells
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- Purification and Some Properties of Lignostilbene-α, β-dioxygenase Isozyme IV from Pseudomonas paucimobilis TMY1009
- Cloning, Expression, and Sequence Analysis of a Lignostilbene-α, β-dioxygenase Gene from Pseudomonas paucimobilis TMY1009
- Cloning of a Lignostilbene-α,β-dioxy-genase Gene from Pseudomonas paucimobilis TMY1009(Microbiology & Fermentation Industry)
- Structural and Enzymatical Comparison of Lignostilbene-α,β-dioxygenase Isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009
- Cloning of a Lignostilbene-α,β-dioxygenase Isozyme Gene from Pseudomonas Paucimobilis TMY1009