Cloning, Expression, and Sequence Analysis of a Lignostilbene-α, β-dioxygenase Gene from Pseudomonas paucimobilis TMY1009

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概要

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• From the genomic library of Pseudomonas paucimobilis TMY1009 constructed with the cosmid pHC79, an 8-kb BamHI-KphI fragment encoding lignostilbene-α, β-dioxygenase (LSD) was cloned into pUC19 (designated pKSN2510). E. coli JM109 having pKSN2510 produced a small amount of LSD only when the lac promoter was induced by isopropyl-β-D-thio-galactopyranoside (IPTG). The 1.9-kb SalI fragment containing the LSD gene on pKSN2510 was subcloned into pUC119 in opposite directions (designated pKHN2560 and pKHN2590). The LSD gene on pKHN2590 was expressed in E. coli MV1184 using the lac promoter. LSD produced by E. coli MV1184 transformed with pKHN2590 (cloned LSD) was purified and found to be identical with LSD-I, which was the major isozyme produced by P. paucimobilis TMY1009. Cloned 1.9-kb nucleotide was sequenced and one open reading frame composed to 486 codons was found.

• 社団法人日本農芸化学会の論文
• 1993-06-23

著者

• SABURI Yoshimasa

  Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, University of

• KAMODA Shigehiro

  Department of Forest Products, Faculty of Agriculture, The University of Tokyo

• Saburi Yoshimasa

  Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences University Of T

• Kamoda Shigehiro

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

関連論文

• Inhibition of Trichoderma cellulase activity by a stilbene glucoside from Picea glehnii bark
• Purification and Some Properties of Lignostilbene-α,β-dioxygenase Responsible for the C_α-C_β Cleavage of a Diarylpropane Type Lignin Model Compound from Pseudomonas sp. TMY1009(Biological Chemistry)
• Purification and Some Properties of Two C_α-dehydrogenases Oxidizing Dimeric Lignin Model Compounds from Pseudomonas sp. TMY1009(Biological Chemistry)
• Production of Heterogeneous Dimer Lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli Cells
• A Common Structure of Substrate Shared by Lignostilbenedioxygenase Isozymes from Sphingomonas paucimobilis TMY1009(Microbiology & Fermentation Technology)
• Purification and Some Properties of Lignostilbene-α, β-dioxygenase Isozyme IV from Pseudomonas paucimobilis TMY1009
• Cloning, Expression, and Sequence Analysis of a Lignostilbene-α, β-dioxygenase Gene from Pseudomonas paucimobilis TMY1009
• Cloning of a Lignostilbene-α,β-dioxy-genase Gene from Pseudomonas paucimobilis TMY1009(Microbiology & Fermentation Industry)
• Structural and Enzymatical Comparison of Lignostilbene-α,β-dioxygenase Isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009
• Cloning of a Lignostilbene-α,β-dioxygenase Isozyme Gene from Pseudomonas Paucimobilis TMY1009

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