Cloning of a Lignostilbene-α,β-dioxygenase Isozyme Gene from Pseudomonas Paucimobilis TMY1009

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概要

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• A genomic DNA library of Pseudomonas paucimobilis TMY1009 was constructed using a cosmid vector pWE15. Screening of the library for lignostilbene-α, β-dioxygenase (LSD) isozyme genes was done with a common probe for the α, β, and γ subunits, which composed LSD isozymes. The positive clones obtained by colony hybridization were further confirmed by Southern hybridization. A 4. 2-kb BamHI-HindIII fragment hybridized with the probe was subcloned into pUC118 to yield the plasmid pKHE1700. Escherichia coli MV1184 carrying pKHE1700 produced one of the LSD isozymes. The cloned LSD was purified and compared with LSD isozymes from P. paucimobilis TMY1009. The behavior on column chromatographies through all purification steps accorded with that of LSD-III. Furthermore' the mobility on polyacrylamide gel electrophoresis, the elution profile on reversed-phase HPLC, and the partial amino acid sequence were common to the cloned LSD and the native LSD-III. Thus the cloned LSD could be identified as LSD-III. It was found that the gene of LSD-III (lsdB) was composed of 489 codons.

• 社団法人日本農芸化学会の論文
• 1995-10-23

著者

• KAMODA Shigehiro

  Department of Forest Products, Faculty of Agriculture, The University of Tokyo

• Saburi Yoshimasa

  Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences University Of T

• Kamoda Shigehiro

  Department Of Forest Products Faculty Of Agriculture The University Of Tokyo

関連論文

• Inhibition of Trichoderma cellulase activity by a stilbene glucoside from Picea glehnii bark
• Purification and Some Properties of Lignostilbene-α,β-dioxygenase Responsible for the C_α-C_β Cleavage of a Diarylpropane Type Lignin Model Compound from Pseudomonas sp. TMY1009(Biological Chemistry)
• Purification and Some Properties of Two C_α-dehydrogenases Oxidizing Dimeric Lignin Model Compounds from Pseudomonas sp. TMY1009(Biological Chemistry)
• Production of Heterogeneous Dimer Lignostilbenedioxygenase II from lsdA and lsdB in Escherichia coli Cells
• A Common Structure of Substrate Shared by Lignostilbenedioxygenase Isozymes from Sphingomonas paucimobilis TMY1009(Microbiology & Fermentation Technology)
• Purification and Some Properties of Lignostilbene-α, β-dioxygenase Isozyme IV from Pseudomonas paucimobilis TMY1009
• Cloning, Expression, and Sequence Analysis of a Lignostilbene-α, β-dioxygenase Gene from Pseudomonas paucimobilis TMY1009
• Cloning of a Lignostilbene-α,β-dioxy-genase Gene from Pseudomonas paucimobilis TMY1009(Microbiology & Fermentation Industry)
• Structural and Enzymatical Comparison of Lignostilbene-α,β-dioxygenase Isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009
• Cloning of a Lignostilbene-α,β-dioxygenase Isozyme Gene from Pseudomonas Paucimobilis TMY1009

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