Cloning and Expression in Escherichia coli of a Gene Coding for a Secondary Alcohol Dehydrogenase from Candida parapsilosis
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概要
- 論文の詳細を見る
A gene encoding a stereo-specific secondary alcohol dehydrogenase (CpSADH) that catalyzed the oxidation of (S)-1,3-BDO to 4-hydroxy-2-butanone was cloned from Candida parapsilosis. This CpSADH-gene consisted of 1,009 nucleotides coding for a protein with M_r 35,964. A recombinant Escherichia coli JM109 strain harboring the expression plasmid, pKK-CPA1,produced (R)-1,3-BDO (93.5%ee, 94.7%yield) from the racemate without any additive to regenerate NAD^+ from NADH.
- 社団法人日本農芸化学会の論文
- 1999-06-23
著者
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KOBAYASHI Yoshinori
Tsukuba Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Kobayashi Y
Daicel Chemical Industries Ltd. Ibaraki Jpn
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Yamamoto H
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Yamamoto Hiroaki
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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Kobayashi Yoshinori
Life Science Development Center Cpi Company Daicel Chemical Industries Ltd. Tsukuba Research Center
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MATSUYAMA Akinobu
Corporate Development Center, Daicel Chemical Industries, Ltd., Tsukuba Research Center
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YAMAMOTO Hiroaki
Tsukuba Research Center, Daicel Chemical Industries, Ltd.
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MATSUYAMA Akinobu
Tsukuba Research Center, Daicel Chemical Industries, Ltd.
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KAWADA Naoki
Research Center, Daicel Chemical Industries, Ltd.
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Kawada Naoki
Research Center Daicel Chemical Industries Ltd.
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Yamashita H
Mathematical Systems Inc.
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Matsuyama A
Corporate Development Center Daicel Chemical Industries Ltd. Tsukuba Research Center
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Kobayashi Yoshinori
Tsukuba Laboratory P.c.c. Technology Inc. C/o Tsukuba Research Laboratories Kyowa Hakko Kogyo Co. Lt
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Yamamoto Hiroaki
Tsukuba Research Center Daicel Chemical Industries Ltd.
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