A Practical Method for Measuring Deoxynivalenol, Nivalenol, and T-2 + HT-2 Toxin in Foods by an Enzyme-linked Immunosorbent Assay Using Monoclonal Antibodies

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We have developed and tested an enzyme-linked immunosorbent assay system for individual measurement of deoxynivalenol, nivalenol, and T-2 + HT-2 toxin using monoclonal antibodies for 3,4,15-triacetyl-nivalenol, for both 3,4,15-triacetyl-nivalenol and 3,15-diacetyl-deoxynivalenol, and for acetyl-T-2 toxin. The assay system comprised three kits (desinated the DON + NIV kit, the NIV kit, and the T-2 + HT-2 kit). The practical performance of the enzyme-linked immunosorbent assay system was assessed by assaying trichothecene mycotoxins in wheat kernels. The enzyme-linked immunosorbent assay system meets all the requirements for use in a routine assay in terms of sensitivity (detection limit: deoxynivalenol 80 ng/g, nivalenol 80 ng/g, T-2 toxin 30 ng/g), reproducibility (total coefficient of variation: 1.9–6.2%), accuracy (recovery: 93.8–112.0%), simplicity and rapidity (time required: <2 h), mass handling (>42 samples/assay), and a good correlation with gas chromatography–mass spectrometry (r=0.9146–0.9991). Components derived from the wheat extract did not interfere with the assay kits. The enzyme-linked immunosorbent assay system is a useful alternative method to gas chromatography–mass spectrometry, liquid chromatography–mass spectrometry, or liquid chromatography–ultraviolet absorption for screening cereals and foods for trichothecene mycotoxin contamination.
社団法人日本農芸化学会の論文
2004-10-23