Isolation of Manganese-containing Superoxide Dismutase from Bacillus subtilis
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概要
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Bacillus subtilis is regarded as one of the most important bacteria in the food industry. Next to Mycobacterium lepraemurium, B. subtilis (IFO 3022) is the best bacterial producer of superoxide dismutase. The B. subtilis superoxide dismutase was purified 40-fold to homogeneity from a cellfree extract using ammonium sulfate fractionation and DEAE-Toyopearl and Sephadex G-150 chromatography. The enzyme has a molecular weight of 45, 000 and consists of two subunits identical in molecular weight. The enzyme, which exhibits absorption maxima at 280 and 470nm, contains 1.13g atoms of Mn per mol of enzyme as the catalytically active metal.
- 社団法人 日本農芸化学会の論文
著者
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Soda Kenji
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Kido Toshiko
Laboratory Of Microbial Biochemistry Institute Of Chemical Research Kyoto University
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TSUKUDA Koji
Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University
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SHIMASUE Yoshiyuki
Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University
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