meso-α, ε-Diaminopimelate D-Dehydrogenase: Sulfhydryl Group Modification

概要

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meso-α, ε-Diaminopimelate D-dehydrogenase was inhibited by sulfhydryl reagents such as p-chloromercuribenzoate and HgCl2. Two sulfhydryl groups were titrated per molecule in the presence and absence of 6 M guanidine hydrochloride: the enzyme contained one sulfhydryl group per subunit. Modification of the sulfhydryl groups with p-chloromercuribenzoate, 5, 5 -dithiobis(2-nitrobenzoic acid), 4, 4-dithiopyridine, N-ethylmaleimide, and iodoacetic acid was accompanied by a loss of enzymeactivity. However, modification of sulfhydryl groups of the enzyme with cyanide did not affect the activity. Thus, the introduction of bulky or charged substituents to sulfhydryl groups decreased the catalytic activity of the enzyme, but modification of the groups with the small and uncharged group, a cyano group, did not. The sulfhydryl groups did not play an essential role in catalysis.
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