Cloning, Sequencing, and Overexpression in Escherichia coli of a Phenylserine Dehydratase Gene from Ralstonia pickettii PS22(Biochemistry & Molecular Biology)

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概要

• 論文の詳細を見る

• The structural gene coding for phenylserine dehydratase from Ralstonia pickettti PS22 was cloned into Escherichia cott cells, and the nucleotide sequence was identified. The predicted amino acid sequence had high sequence similarity to biodegradative and biosynthetic threonine dehydratases from E. coli and serine dehydratase from human liver. Transformed E. coli cells overproduced phenylserine dehydratase, and the recombinant enzyme was purified to homogeneity with a high yield and characterized.

• 社団法人日本農芸化学会の論文
• 2002-12-23

著者

• MISONO Haruo

  Department of Bioresources Science, Kochi University

• Meguro H

  Tohoku Fukushi University

• Meguro H

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Misono H

  Kochi Univ. Kochi Jpn

• Misono Haruo

  Department Of Bioresources Science Faculty Of Agriculture Kochi University

• Misono Haruo

  Department Of Bioresource Science Kochi University

• Misono Haruo

  Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kochi University

• NAGATA Shinji

  Department of Internal Medicine, Hiroshima City Asa Hospital

• OKUDA Heiwa

  Department of Bioresources Science, Kochi University

• Nagata Shinji

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Meguro Hiroshi

  Kansei-fukushi Research Center Tohoku Fukushi University

• Nagasaki S

  Department Of Bioresources Science Kochi University

• Moriyama Hideaki

  Japan Synchrotron Radiation Research Institute (JASRI)

• Okuda H

  Department Of Bioresources Science Kochi University:(present Address)department Of Urology Kochi Med

• Moriyama H

  Department Of Life Science Faculty Of Biocscience And Bioctechnology Tokyo Institute Of Technology

• NAGATA Shinji

  Department of Agricultural Chemistry, Kochi University

• MISONO Haruo

  Department of Agricultural and Biological Chemistry, Kochi University

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