Leucine Dehydrogenase from Corynebacterium pseudodiphtheriticum : Purification and Characterization(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Leucine dehydrogenase [EC 1.4.1.9] was purified to homogeneity from Corynebacterium pseudodiphtheriticum ICR 2210. The enzyme consisted of a single polypeptide with a molecular weight of about 34,000. Stepwise Edman degradation provided the N-terminal sequence of the first 24 amino acids, and carboxypeptidase Y digestion provided the C-terminal sequence of the last 2 amino acids. Although the enzyme catalyzed the reversible deamination of various branched-chain L-amino acids, L-valine was the best substrate for oxidative deamination at pH 10.9 and the saturated concentration. The enzyme, however, had higher reactivity for L-leucine, and the k_<cat>/km value for L-leucine was higher than that for L-valine. The enzyme required NAD^+ as a natural coenzyme. The NAD^+ analogs 3-acetylpyridine-NAD^+ and deamino-NAD^+ were much better coenzymes than NAD^+. The enzyme activity was significantly reduced by sulfhydryl reagents and pyridoxal 5'-phosphate. D-Enantiomers of the substrate amino acids competitively inhibited the oxidation of L-valine.
- 社団法人日本農芸化学会の論文
- 1990-06-23
著者
-
Meguro H
Tohoku Fukushi University
-
Meguro H
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
-
Misono Haruo
Department Of Bioresources Science Faculty Of Agriculture Kochi University
-
Misono Haruo
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kochi University
-
NAGATA Shinji
Laboratory of Bioorganic Chemistry, Department of Applied Biological Chemistry, Graduate School of A
-
Nagasaki S
Department Of Bioresources Science Kochi University
-
Nagata Shinji
Laboratory Of Applied Microbiology Department Of Bioresources Science Kochi University
-
Nagasaki Susumu
Laboratory of Applied Microbiology, Department of Bioresources Science, Kochi University
-
SUGIHARA Kouji
Laboratory of Applied Microbiology, Department oj Agricultural Chemistry, Kochi University
-
KUWAMOTO Yumiko
Laboratory of Applied Microbiology, Department oj Agricultural Chemistry, Kochi University
-
Moriyama Hideaki
Japan Synchrotron Radiation Research Institute (JASRI)
-
Sugihara Kouji
Laboratory Of Applied Microbiology Department Oj Agricultural Chemistry Kochi University
-
Kuwamoto Yumiko
Laboratory Of Applied Microbiology Department Oj Agricultural Chemistry Kochi University
関連論文
- Highly Sensitive Flow Injection Analysis of Lipid Hydroperoxides in Foodstuffs
- Determination of the Absolute Configurations of the Anteiso Acid Moieties of Glycoglycerolipid S365A Isolated from Corynebacterium aquaticum(Analytical Chemistry)
- Absolute Configuration of a Ceramide with a Novel Branched-chain Fatty Acid Isolated from the Epiphytic Dinoflagellate, Coolia monotis
- Highly Sensitive Isomeric Determination of Beraprost Sodium in Plasma Using a Fluorescent Chiral Derivatization Reagent (Proceedings of Asianalysis 4--The Fourth Asian Conference on Analytical Sciences)
- Automatic determination of hydroperoxides of phosphatidylcholine and phosphatidylethanolamine in human plasma
- Enzymatic Assay of Phosphatidylcholine Hydroperoxide by Phospholipase A_2 and Glutathione Peroxidase-Based on Fluorometry with N-(9-Acridinyl)maleimide
- Fluorometric Assay of Glutathione Peroxidase Activity in Liver and Plasma with N- (9-Acridinyl)maleimide
- Determination of Dinophysis Toxin-3 by the LC-LC Method with Fluorometric Detection
- Crystal Structure of H_2-Proteinase from the Venom of Trimeresurus flavoviridis
- Ligand-Induced Changes in the Conformation of 3-Isopropylmalate Dehydrogenase from Thermus thermophilus
- Crystallization and Preliminary X-Ray Studies of Isocitrate Dehydrogenase from Thermus thermophilus HB8
- Crystallization and Preliminary X-Ray Study of H_2-Proteinase from the Venom of Trimeresurus flavoviridis
- The Crystal Structures of Mutated 3-Isopropylmalate Dehydrogenase from Thermus thermophilus HB8 and Their Relationship to the Thermostability of the Enzyme
- Cloning and Sequencing of the Serine Dehydrogenase Gene from Agrobacterium tumefaciens(Biochemistry & Molecular Biology)
- Enzymatic Synthesis of L-Serine from D-Glycerate(Microbiology & Fermentation Industry)
- Potential D, L-Amino Acid Sequence Analysis of Peptides from the C-Terminus
- Simultaneous Analysis of Enantiomeric Amino Acids Based on Derivatization with(S)-(+)-2-t-Butyl-2-methyl-1,3-benzodioxole-4-carboxylic Acid and High-Performance Liquid Chromatography
- A New Reaction of Adenine with a 1,4-Anhydroribitol Having a Vicinal cis-Oriented Azide and O-Tosylate: Formation of a Novel Aziridinoadenine Isonucleoside
- Synthesis of 4'-C-Methylnucleosides
- Synthesis of Conceptually New, Potentially Anti-viral, Low-Toxity, Acid- and Enzyme-stable Furanoid and Pyranoid Nucleosides
- Simple Method to Determine the Absolute Configuration of the Glycerol Moiety in Glycosyl Glycerols Based on ORD and CD(Organic Chemistry)
- Synthesis and Characterization of Chirally Deuteriated sn-Glycerols(Organic Chemistry)
- Synthesis of N-Acetyl-3-fluoro-neuraminic Acids(Organic Chemistry)
- Syntheses and ^1H-NMR Studies of Methyl 4,6-Di-O-glucopyranosyl-β-D-glucopyranosides Chirally Deuterated at the (1→6)-Linkage Moiety(Organic Chemistry)
- Conformational Analysis of Mucin-type Sugars by ^1H-NMR Spectroscopy of the Chirally Deuterated Analogs(Organic Chemistry)
- Syntheses of Chiral γ-Lactones from D- and L-Arabinoses(Organic Chemistry)
- Synthesis of (4S,5S)-4,5-Dihydroxy-4,5-O-isopropylidene-2-cyclopenten-1-one from D-Ribose(Organic Chemistry)
- Chiral Deuteration at C-5 of 1,5-Anhydropentofuranose Derivatives(Organic Chemistry)
- Syntheses and Flavors of Four Possible Optical Isomers of Marmelo Lactones
- PRIMARY STRUCTURE OF RATFISH PROOPIOMELANOCORTIN
- Mass spectrometric detection of proopiomelanocortin (POMC)-related peptides following molecular cloning of POMC cDNA in bigeye tuna Thunnus obesus
- Cloning and Expression of Endo-β-1,3-glucanase gene from Flavobacterium dormitatov in Escherichia coli and Characterization of the Gene Product(Biological Chemistry)
- Neoculin as a New Taste-modifying Protein Occurring in the Fruit of Curculigo latifolia
- Syntheses of a Series of Fluorescent Carboxylic Acids with a 1, 3-Benzodioxole Skeleton and Their Evaluation as Chiral Derivatizing Reagents
- Cloning, Sequencing, and Overexpression in Escherichia coli of a Phenylserine Dehydratase Gene from Ralstonia pickettii PS22(Biochemistry & Molecular Biology)
- A Novel Phenylserine Dehydratase from Pseudomonas pickettii PS22: Purification, Characterization, and Sequence of Its Phosphopyridoxyl Peptide
- Measurement of Cholesterol Ester Hydroperoxides of High and Combined Low and Very Low Density Lipoprotein in Human Plasma
- Measurement of Hydroxy and Hydroperoxy Fatty Acids by a High Pressure Liquid Chromatography with a Column-switching System
- A Simple Fluorometry of Hydroperoxides in Oils and Foods
- A Sensitive Enzymatic Determination of Acylglycerol Hydroperoxides by Lipase and Glutathione Peroxidase with N-(9-Acrydinyl)maleimide Fluorometry(Analytical Chemistry)
- A Sensitive Enzymatic Determination of Fatty Acid Hydroperoxide by Glutathione Peroxidase with N-(9-Acrydinyl)maleimide Fluorometry(Analytical Chemistry)
- Fluorometric Determination of Sulfite in Wine by N-(9-Acridinyl)maleimide(Analytical Chemistry)
- Fluorometric Determination of Sulfite with N-(9-Acridinyl)maleimide for High Performance Liquid Chromatography(Analytical Chemistry)
- Cloning and Overexpression of the 3-Hydroxyisobutyrate Dehydrogenase Gene from Pseudomonas putida E23(Biochemistry & Molecular Biology)
- A Novel NADP^+-dependent Serine Dehydrogenase from Agrobacterium tumefaciens
- 3-Hydroxyisobutyrate Dehydrogenase from Pseudomonas putida E23: Purification and Characterization
- Selective Determination of L-Lysine with L-Lysine ε-Dehydrogenase(Analytical Chemistry)
- Stereospecificity of Hydrogen Transfer of the Coenzyme Catalyzed by L-Lysine ε-Dehydrogenase(Biological Chemistry)
- Occurrence of an Inducible Phenylserine Dehydratase in Pseudomonas pickettii PS22 Isolated from Soil
- Occurrence of an Inducible NADP^ -Dependent D-Phenylserine Dehydrogenase in Pseudomonds syringae NK-15 Isolated from Soil
- Jack Bean β-N-Acetylhexosaminidase Does Not Hydrolyze N-Acetylchitooligosacchar-ides
- Purification and Characterization of Nonlytic Endo-β-1,3-Glucanase I from Flavobacterium dormitator var. glucanolyticae(Biological Chemistry)
- Leucine Dehydrogenase from Corynebacterium pseudodiphtheriticum : Purification and Characterization(Biological Chemistry)
- Gene Clonign, Purification, and Characterization of the Highly Thermostable Leucine Dehydrogenase of Bacillus sp.
- Gene Cloning, Purification and Characterization of Thermostable Alanine Dehydrogenase of Bacillus stearothermophilus
- New Antibiotic-Producing Streptomyces TT-Strain, Generated by Electrical Fusion of Protoplasts
- Interspecific Electrofusion between Protoplasts of Streptomyces antibioticus and Streptomyces fradiae(Microbiology & Fermentation Industry)
- Leucine Dehydrogenase of an Thermophilic Anaerobe, Clostridium thermoaceticum: Gene Cloning, Purification and Characterization(Microbiology & Fermentation Industry)
- Characterization of meso-Diaminopimelate Dehydrogenase from Corynebacterium glutamicum and Its Distribution in Bacteria(Microbiology & Fermentaion Industry)
- Purification and Properties of meso-Diaminopimelate Dehydrogenase from Brevibacterium sp.
- Characteristics of Enzymes Participating in D-Amino Acids Metabolism(Chiral Homogeneity and D-Amino Acids, International Symposium on Origins of Life and Astrobiology)
- Characterization of yrpC Gene Product of Bacillus subtilis IFO 3336 as Glutamate Racemase Isozyme
- Properties of Glutamate Racemase from Bacillus subtilis IFO 3336 Producing Poly-γ-Glutamate^1
- Prospects for X-ray Crystal Structure Analysis of Selenoproteins with SPring-8 Synchrotron Radiation
- Cloning of Alanine Racemase Genes from Pseudomonas fluorescens Strains and Oligomerization States of Gene Products Expressed in Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Directed Evolution of Bacterial Alanine Racemases with Higher Expression Level(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Directed Evolution of Bacterial Alanine Racemases with Higher Expression Level
- Molecular Breeding of 2, 3-Dihydroxybiphenyl 1, 2-Dioxygenase for Enhanced Resistance to 3-Chlorocatechol
- Alanine Dehydrogenase from Enterobacter aerogenes : Purification, Characterization, and Primary Structure
- Study on the Application of the (S)-2-tert-Butyl-2-methyl-1,3-benzodioxole-4-carboxylic acid-based On-line HPLC-Exciton CD Analysis to Acyclic Vicinal Diols and 1,3-Diols
- Purification, Crystallization and Properties of NADP+-Specific Glutamate Dehydrogenase from Lactobacillus fermentum
- Correlation between catalytic activity and monomer-dimer equilibrium of bacterial alanine racemases
- meso-α, ε-Diaminopimelate D-Dehydrogenase: Sulfhydryl Group Modification
- Purification and Properties of meso-α, ε-Diaminopimelate Decarboxylase from Bacillus sphaericus