Fluorometric Determination of Sulfite with N-(9-Acridinyl)maleimide for High Performance Liquid Chromatography(Analytical Chemistry)
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概要
- 論文の詳細を見る
The fluorometric reaction of sulfite with N-(9-acridinyl)maleimide (NAM) was applied to high performance liquid chromatography (HPLC). It improved the sensitivity and accuracy for the determination of sulfite to 0.01 nmol/ml or 0.1 pmol on the column. Three NAM-sulfite adducts were isolated, and their structures were identified as N-(9'-acridinyl)-2-sulfonyl succinimide (I) and its two isomeric hydrolysates, N-(9'-acridinyl)-2 or -3-sulfonyl succinamides (II and II'). Two-step reactions, involving the addition of sulfite to the maleimide ring of NAM and subsequent hydrolysis of the resulting succinimide ring to two amide-form adducts, were established.
- 社団法人日本農芸化学会の論文
- 1986-05-23
著者
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大類 洋
横浜薬科大学
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大類 洋
東北大・農
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Meguro H
Tohoku Fukushi University
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Meguro H
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Ohrui Hiroshi
Department Of Biological Chemistry Faculty Of Agriculture Tohoku University
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SUZUKI Tateo
Laboratory of Analytical Chemistry, Faculty of Agriculture, Tohoku University
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MEGURO Hiroshi
Laboratory of Analytical Chemistry, Faculty of Agriculture, Tohoku University
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Suzuki T
Tokyo Univ. Fisheries Tokyo Jpn
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Suzuki Tateo
Laboratory Of Analytical Chemistry Faculty Of Agriculture Tohoku University
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Suzuki Toshihiko
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Shizuoka University
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AKASAKA Kazuaki
Laboratory of Analytical Chemistry, Faculty of Agriculture, Tohoku University
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OHRUI Hiroshi
Laboratory of Analytical Chemistry, Faculty of Agriculture, Tohoku University
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Suzuki T
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Shizuoka University
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Meguro Hiroshi
Laboratory Of Analytical Chemistry Faculty Of Agriculture Tohoku University
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赤坂 和昭
東北大 大学院
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