A Novel NADP^+-dependent Serine Dehydrogenase from Agrobacterium tumefaciens
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概要
- 論文の詳細を見る
NADP-dependent serine dehydrogenase [EC 1.1.1.-], which catalyzes the oxidation of the hydroxyl group of serine to form 2-aminomalonate semialdehyde, was purified to homogeneity from a crude extract of Agrobacterium tumefaciens ICR 1600. The enzyme had a molecular mass of about 100 kDa and consisted of four identical subunits. In addition to L-serine, D-serine, L-glycerate, D-glycerate, and 2-methyl-DL-serine were substrates. However, O-methyl-DL-serine and L-threonine were inert. The enzyme showed maximal activity at about pH 9 for the oxidation of L-serine. The enzyme required NADP as a coenzyme, NAD was inert. The enzyme was not inhibited by EDTA, o-phenanthroline, or α, α'-dipyridyl, but was inhibited by HgCI_2, p-chloromercuribenzoate, L-cysteine, D-cysteine, malonate, 2-methylmalonate, and tartronate. The Michaelis constants for L-serine, D-serine, and NADP were 42, 44, and 0.029 mM, respectively.
- 社団法人日本農芸化学会の論文
- 1997-01-23
著者
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Meguro H
Tohoku Fukushi University
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Chowdhury E
Department Of Bioresources Science Kochi University:(present Address)department Of Biochemistry Univ
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Misono H
Kochi Univ. Kochi Jpn
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Misono Haruo
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Misono Haruo
Department Of Bioresource Science Kochi University
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Misono Haruo
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kochi University
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NAGATA Shinji
Laboratory of Bioorganic Chemistry, Department of Applied Biological Chemistry, Graduate School of A
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Chowdhury Emran
Laboratory of Applied Microbiology, Department of Bioresources Science, Kochi University
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Higuchi Kazuhiko
Laboratory of Applied Microbiology, Department of Bioresources Science, Kochi University
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Nagata S
Department Of Bioresources Science Kochi University
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Nagata Shinji
Laboratory Of Applied Microbiology Department Of Bioresources Science Kochi University
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Higuchi Kazuhiko
Laboratory Of Applied Microbiology Department Of Bioresources Science Kochi University
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