Interspecific Electrofusion between Protoplasts of Streptomyces antibioticus and Streptomyces fradiae(Microbiology & Fermentation Industry)

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概要

• 論文の詳細を見る

• The interspecific electrofusion between protoplasts of Streptomyces antibioticus a20 (Ala^-) and S. fradiae f2 (His^-) was examined. The pulse intensities required for the breakdown of half of the protoplasts were 7 kV/cm for S. antibioticus a20 and 5 kV/cm for S. fradiae f2. The optimum pulse intensity for the fusion was found to be 6 kV/cm. When the density of a protoplast suspension was 1 × 10^8 protoplasts/ml in a 0.3 M sucrose solution containing 2.5mM MgCl_2 and CaCl_2, the maximum fusion frequency of 2.7% was obtained on the application of one square field pulse of 6 kV/cm for 20 μsec after dielectrophoresis in an alternating field of 800 V/cm (1 MHz) for 60 sec. The fusion frequency was much higher than that obtained on polyethyleneglycol-induced fusion (0.15%). This is the first case of electrofusion of Streptomyces protoplasts, which are as small as those of some bacteria.

• 社団法人日本農芸化学会の論文
• 1988-06-23

著者

• MISONO Haruo

  Department of Bioresources Science, Kochi University

• Meguro H

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Misono Haruo

  Department Of Bioresource Science Kochi University

• NAGATA Shinji

  Department of Internal Medicine, Hiroshima City Asa Hospital

• Nagata Shinji

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• NAGASAKI Susumu

  Department of Agricultural Chemistry, Kochi University

• Nagasaki S

  Department Of Bioresources Science Kochi University

• OKAMURA TOKUMITSU

  Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Kochi, University

• Moriyama Hideaki

  Japan Synchrotron Radiation Research Institute (JASRI)

• Okamura T

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Okamura Tokumitsu

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Nagasaki Susumu

  Department Of Agricultural Chemistry Faculty Of Agriculture Kochi University

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