Cloning and Overexpression of the 3-Hydroxyisobutyrate Dehydrogenase Gene from Pseudomonas putida E23(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The structural gene for NAD^+-dependent 3-hydroxyisobutyrate dehydrogenase (EC 1. 1. 1. 31) from Pseudomonas putida E23 was cloned in Escherichia coli cells to obtain a large amount of the enzyme and its nucleotides were sequenced to study its structural relationship with other proteins. The gene encoded a polypeptide containing 295 amino acid residues and was in a cluster with the gene for methylmalonate semialdehyde dehydrogenase. Transformed E. coli cells overproduced 3-hydroxyisobutyrate dehydrogenase, and the recombinant enzyme was purified to homogeneity with a high yield. Lysine and asparagine residues, which are important in catalysis of the 3-hydroxyacid dehydrogenase family, are conserved in this enzyme.
- 社団法人日本農芸化学会の論文
- 2003-02-23
著者
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MISONO Haruo
Department of Bioresources Science, Kochi University
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Meguro H
Tohoku Fukushi University
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Chowdhury E
Department Of Bioresources Science Kochi University:(present Address)department Of Biochemistry Univ
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Misono H
Kochi Univ. Kochi Jpn
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Misono Haruo
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Misono Haruo
Department Of Bioresource Science Kochi University
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Misono Haruo
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kochi University
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NAGATA Shinji
Department of Internal Medicine, Hiroshima City Asa Hospital
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CHOWDHURY Emran
Department of Bioresources Science, Kochi University
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Chowdhury Emran
Department Of Bioresources Science Kochi University:(present Address)department Of Biochemistry Univ
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AKAISHI Yuka
Department of Bioresources Science, Kochi University
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Akaishi Yuka
Department Of Bioresources Science Kochi University
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Nagata S
Department Of Bioresources Science Kochi University
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Nagata Shinji
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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NAGATA Shinji
Department of Agricultural Chemistry, Kochi University
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MISONO Haruo
Department of Agricultural and Biological Chemistry, Kochi University
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