New Antibiotic-Producing Streptomyces TT-Strain, Generated by Electrical Fusion of Protoplasts
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概要
- 論文の詳細を見る
Interspecific electrofusion between protoplasts of multhiomycin-producing Streptomyces antibioticus aAL Ala〓Leu〓 and neomycin-producing S. fradiae fHL His〓Leu〓 was done. When the concentration of both protoplasts increased to 1×10^9 protoplasts/ml, the frequency of fusion attained was 18.6%. The addition of multhiomycin and neomycin to the regeneration medium was very effective for screening for fusants able to produce new antibiotics. The ability to produce new antibiotics was very unstable in the fusants. After several subcultures, fusants selected as new antibiotic producers (10 strains) lost this ability with one exception (TT-strain). The antibiotic produced by the stable TT-strain clone was purified and characterized to some extent. It was active on a range of Gram-positive bacteria and distinguished from multhiomycin and neomycin by bioautographic analysis.
- 社団法人日本生物工学会の論文
- 1989-04-25
著者
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Meguro H
Tohoku Fukushi University
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Meguro H
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Misono H
Kochi Univ. Kochi Jpn
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Misono Haruo
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Misono Haruo
Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Kochi University
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NAGATA Shinji
Laboratory of Bioorganic Chemistry, Department of Applied Biological Chemistry, Graduate School of A
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Nagasaki S
Department Of Bioresources Science Kochi University
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Nagata Shinji
Laboratory Of Applied Microbiology Department Of Bioresources Science Kochi University
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Nagasaki Susumu
Laboratory of Applied Microbiology, Department of Bioresources Science, Kochi University
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OKAMURA TOKUMITSU
Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Kochi, University
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Moriyama Hideaki
Japan Synchrotron Radiation Research Institute (JASRI)
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Okamura T
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Okamura Tokumitsu
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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