An Improved Convenient Molecular Weight-determination Method for Active Stainable-Enzyme after SDS Electrophoresis
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概要
- 論文の詳細を見る
An improved method to determine the molecular weight of alcohol dehydrogenase after sodium dodecylsulfate polyacrylamide gel electrophoresis(SDS-PAGE) has been developed. This method was based on the finding that on a gel, which was washed with a buffer to remove SDS after SDS-PAGE, stained with an enzymatic activity staining mixture and then stained with coomassie blue, there appeared two active stained bands with apparent molecular weights of 148,000(tetramer)and 35,000(monomer)on the SDS-PAGE gel. The method developed here may be applicable to a wide range of active stainable-enzymes as a rapid and simple molecular weight determination method after SDS-PAGE.
- 武庫川女子大学の論文
著者
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Ohsugi M
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Horie Noboru
School Of Human Environmental Science Mukogawa Women's University
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OHSUGI Masahiro
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's U
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Horie Noboru
Department Of Food Science And Nutrition School Of Home Life And Environmental Sciences Mukogawa Wom
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Okamura T
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Okamura Tokumitsu
Department Of Food Science And Nutrition School Of Home Life And Environmental Science Mukogawa Wome
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Okamura Tokumitsu
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Ohsugi Masahiro
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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OHSUGI Masahiro
Department of Domestic Science, Mukogawa Womens University
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