Isolation and Culture of Protoplasts from Gymnocalycium baldianum (speg.) Speg. (Hikagyoku) Callus Cultures

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Isolation and culture of protoplasts from Gymnocalycium baldianum (Hikagyoku) callus cultures were examined. The peeling treatment of fruit largely excluded contamination during the induction and culture of callus, leading to successful formation of callus. Callus was induced effectively from the flesh using MS solid medium containing 0.01μM of kinetin and 0.01μM of 2, 4-dichlorophenoxyacetic acid (2, 4-D). The callus growth in the medium was largely promoted without causing a browning of the callus in the subculture. In preparation of protoplasts, the enzyme solution gave a high yield of protoplasts. Cell division into 2 cells was observed after 24 hours of culture, then micro colonies formed after 7 days, followed by colony formation within 2 weeks.
武庫川女子大学の論文