An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis
スポンサーリンク
概要
- 論文の詳細を見る
An improved method to determine the molecular weight of the subunit of lactate dehydrogenase and malate dehydrogenase after sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed. This method was based on the finding that on a gel, which was washed with a buffer to remove SDS after SDS-PAGE, stained with an enzymatic activity staining mixture and then stained with coomassie blue, there appeared one active stained band with apparent molecular weights of 35,500 (monomer) (lactate dehydrogenase) and 31,000 (monomer) (malate dehydrogenase) on the SDS-PAGE gel. The method developed here may be applicable to a wide range of active stainable-enzymes as a rapid and simple molecular weight determination method of the subunit after SDS-PAGE.
- 武庫川女子大学の論文
著者
-
Ohsugi M
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
-
Horie Noboru
School Of Human Environmental Science Mukogawa Women's University
-
OHSUGI Masahiro
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's U
-
Horie Noboru
Department Of Food Science And Nutrition School Of Home Life And Environmental Sciences Mukogawa Wom
-
Okamura T
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
-
Okamura Tokumitsu
Department Of Food Science And Nutrition School Of Home Life And Environmental Science Mukogawa Wome
-
Okamura Tokumitsu
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
-
Ohsugi Masahiro
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
-
Holie Noboru
Department of Food Science and Nutrition, School of Home Life and Environmental Sciences, Mukogawa Women's University
-
OHSUGI Masahiro
Department of Domestic Science, Mukogawa Womens University
関連論文
- Isolation of Plasma Kallikrein by High Efficiency Affinity Chromatography and Its Characterization
- Fermented Soybean with Thrombosis Preventing Activity Using Mushroom Mycelia as Microbial Source
- Characteristics of Wine Produced by Mushroom Fermentation(Microbiology & Fermentation Technology)
- Utilization of Enokitake and Shiitake in fiber-bread processing and their characteristics
- Callus Induction from Hibiscus manihot Seed and Leaf
- Callus Formation from Eucommia ulmoides (Tu-chung) Branch
- Preparation of Highly Efficient Resin for Affinity Chromatography Employing Synthetic Specific Inhibitors : Further Studies on Purification of Plasma Kallikrein (PK)
- Purification of Plasma Kallikrein (PK) : Preparation of PK Specific Inhibitor-Polymer Conjugate and Its Application to Purification of PK
- Synthesis of 2(1H)-Pyrazinone Derivatives from Dipeptides and Its Application to the Development of Proteinase Inhibitor
- Characteristics of a Cheese-Like Food Produced by Fermentation of the Mushroom Schizophyllum commune
- Characteristics of Beer-Like Drink Produced by Mushroom Fermentation
- Development of Mushrooms for Thrombosis Prevention by Protoplast Fusion
- Dielectrophoresis Conditions for Pearl Chain Formation and Effect of Pulse Field Strength on Protoplast Breakdown of Hericium erinaceum
- Effect of Culture Broths from Various Microorganisms on Fruiting of Pleurotus ostreatus
- The Artificial Cultivation of Collybia spp. W0003
- New Antibiotic-Producing Streptomyces TT-Strain, Generated by Electrical Fusion of Protoplasts
- Interspecific Electrofusion between Protoplasts of Streptomyces antibioticus and Streptomyces fradiae(Microbiology & Fermentation Industry)
- Utilization and characteristics of starch in bread processing
- Production of Betalains by Suspension Cultures of Basella rubra L.
- Induction of Callus from Crataegus cuneata Stems
- Plant Regeneration from Hypocotyl-derived Calli of Gardenia jasminoides
- Effects of Hormones on Cultivation of Tricholoma matsutake Mycelia
- Effect of culture broths from various microorganisms on fruiting of Pleurotus ostreatus W0001
- Cultural characteristics of Laetiporus sulphureus, producing an anti-thrombin substance
- Effects of Aspartame on alcohol fermentation in Aspartame-added bread processing
- Screening of Fibrinolytic Enzymes of Microorganisms
- Screening of Amine Dehydrogenase of Microorganisms
- Screening of Alcohol Dehydrogenase and Amylase of Microorganisms
- Screening of Lactate Dehydrogenase and Curd Rennet of Microorganisms
- An Improved Convenient Molecular Weight-determination Method for Active Stainable-Enzyme after SDS Electrophoresis
- An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis
- Isolation and Culture of Protoplasts from Gymnocalycium baldianum (speg.) Speg. (Hikagyoku) Callus Cultures
- Callus Formation and Plant Regeneration from Basella rubra Leaf and Stem Cultures
- Isolation and Fusion of Protoplasts from Basella rubra Leaf and Stem Cultures
- Growth Stimulation of Bifidobacteria by a Mixed Culture with Vitamin B_-Producing Klebsiella pneumoniae IFO 13541
- Effects of Fermentable Sugar Derived from Bunashimeji Mushroom on Fermentation in Bread Processing
- Application to Food of Lentinus edodes Flour with Extrusion Cooking
- Screening of aspartate dehydrogenase of bacteria
- Fumaric Acid, Anti-Thrombin Substance from Rhizopus javanicus
- Aspartate Dehydrogenase in Vitamin B12-Producing Klebsiella pneumoniae IFO 13541
- D-Glucose Oxidation by Micrococcus sp. Grown on Acetate
- Microbial Degradation of a-Picolinic Acid, 2-Pyridinecarboxylic Acid