Isolation of Plasma Kallikrein by High Efficiency Affinity Chromatography and Its Characterization

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概要

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• We established a simple method for the purification of human plasma kallikrein (PK) by affinity chromatography and characterized it by analytical reverse phase-HPLC and Time of Flight Mass Spectroscopy (TOF-MS). The affinity resin (PKSI-Toyopearl) was synthesized using a selective synthetic inhibitor of plasma kallikrein (PKSI-527) as a ligand. The resin was found to have the highest efficiency in PK purification when the coupling ration of PKSI-527 per resin was 9-14 μmol/g. PK was purified 466-fold with a yield of 83% from acetone-activated human plasma by affinity chromatography. The purity of PK thus obtained was confirmed by reverse phase-HPLC with a linear gradient of acetonitrile. The molecular weight of the purified PK was determined to be 86, 151 by TOF-MS.

• 公益社団法人日本薬学会の論文
• 2001-05-01

著者

• TSUDA Yuko

  Faculty of Pharmaceutical Sciences, Kobe Gakuin University

• OKADA Yoshio

  Faculty of Pharmaceutical Sciences, Kobe Gakuin University

• Yokoi T

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Tsuda Yuko

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University:cooperative Research Center Of Life Scienc

• Tsuda Y

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Tsuda Yuko

  Faculty Of Pharmaceutical Science Kobe Gakuin University

• Okada Yoshio

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Hijikata-Okunomiya Akiko

  Faculty of Health Science, Kobe University School of Medicine

• Okamoto Shosuke

  Kobe Research Projects on Thrombosis and Haemostasis

• Takahashi M

  Research Center For Medicinal Plant Resources National Institute Of Biomedical Innovation

• TADA Mayako

  Faculty of Pharmaceutical Sciences, Kobe Gakuin University

• WANAKA Keiko

  Kobe Research Projects on Thrombosis and Haemostasis

• OKAMOTO Utako

  Kobe Research Projects on Thrombosis and Haemostasis

• HORIE Noboru

  School of Human Environmental Science, Mukogawa Women's University

• Hijikata‐okunomiya A

  Faculty Of Health Science Kobe University School Of Medicine

• Hijikata-okunomiya Akiko

  Faculty Of Health Science Kobe University School Of Medicine

• Okamoto Shosuke

  Kobe Research Projects On Thrombosis And Haemostatis

• Wanaka Keiko

  Kobe Research Projects On Thrombosis And Haemostatis

• Okada Y

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Tada M

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Horie Noboru

  School Of Human Environmental Science Mukogawa Women's University

• Horie N

  School Of Human Environmental Science Mukogawa Women's University

• Tsuda Y

  Faculty Of Pharmaceutical Sciences And High Technology Research Center Kobe Gakuin University

• 岡本 成羽

  Molecular Chemistry Laboratory Takeda Chemical Industries Ltd.

• Ogino Y

  Teikyo Univ. School Of Medicine Chiba

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