Fate of Orally Administered ^15N-LabeIed Polyamines in Rats Bearing Solid Tumors

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We studied absorption, distribution, metabolism, and excretion of polyamines (putrescinc, spermidine, and spermine) in the gastrointestinal tract using ^15N-Iabeled polyamines as tracers and ionspray ionization mass spectrometry (IS-MS). The relatively simple protocol using rats bearing solid tumors provided useful information. Three ^15N-labeIed polyamines that were simultaneously administered were absorbed equally from gastrointestinal tract, and distributed within tissues at various concentrations. The uptake of ^15N-spermidine seemed preferential to that of ^15N-spermine since the concentrations of ^15N-spermidine in the liver and tumors were higher, whereas those of ^15N-spermine were higher in the kidney, probably due to the excretion of excess extracellular spermine. Most of the absorbed ^15N-putrescine seemed to be lost, suggesting blood and tissue diamine oxidase degradation. Concentrations of ^15N-spermidine and ^l5N-spermine in the tumor were low. We also describe the findings from two rats that were administered with ^15N-spermine. The tissue concentrations of ^15N-spermine were unusually high, and significant levels of ^15N-spermidine were derived from ^15N-spermine in these animals.
公益社団法人日本薬学会の論文
2003-03-01