Purification Method for Human Plasma Kallikrein by a New Affinity Chromatography
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概要
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We synthesized a new affinity gel (PKSI-Toyopearl) using a selective synthetic inhibitor of plasma kallikrein (PKSI-527) as an affinity ligand, and employed it for the rapid purification of plasma kallikrein from human plasma. Human plasma activated with kaolin after acid treatment was applied to a PKSI-Toyopearl column. Adsorbed protein was eluted with 50 mM glycine-hydrochloric acid buffer (pH 3.0). Plasma kallikrein was purified 181-fold with a yield of 85% from the kaolin-activated plasma. Further purification was performed by chromatography on a DEAE-Toyopearl 650M column. Plasma kallikrein was finally purified 1720-fold with a 63% yield by these procedures. On sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, a band was observed at approximately 88 kDa. These findings indicate that PKSI-Toyopearl is a valuable tool for the purification of plasma kallikrein from human plasma.
- 公益社団法人日本薬学会の論文
- 1998-02-15
著者
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TSUDA Yuko
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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OKADA Yoshio
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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Nagamatsu Y
Kobe‐gakuin Univ. Kobe Jpn
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Tsuda Yuko
Faculty Of Pharmaceutical Science Kobe Gakuin University
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Okada Yoshio
Faculty Of Pharmaceutical Sciences Kobe Gakuin University
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Hijikata-Okunomiya Akiko
Faculty of Health Science, Kobe University School of Medicine
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Okamoto Shosuke
Kobe Research Projects on Thrombosis and Haemostasis
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Takahashi M
Research Center For Medicinal Plant Resources National Institute Of Biomedical Innovation
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TADA Mayako
Faculty of Pharmaceutical Sciences, Kobe Gakuin University
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WANAKA Keiko
Kobe Research Projects on Thrombosis and Haemostasis
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OKAMOTO Utako
Kobe Research Projects on Thrombosis and Haemostasis
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Hijikata‐okunomiya A
Faculty Of Health Science Kobe University School Of Medicine
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Hijikata-okunomiya Akiko
Faculty Of Health Science Kobe University School Of Medicine
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Okamoto Shosuke
Kobe Research Projects On Thrombosis And Haemostatis
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Wanaka Keiko
Kobe Research Projects On Thrombosis And Haemostatis
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TADA Mayako
Kobe Research Projects on Thrombosis and Haemostasis
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NAKAYA Yukiko
Kobe Research Projects on Thrombosis and Haemostasis
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HORIE Noburo
Department of Food Science and Nutrition, School of Human Environmental Science, Mukogawa Women's Un
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Nakaya Y
Kobe Research Projects On Thrombosis And Haemostasis
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Horie N
School Of Human Environmental Science Mukogawa Women's University
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Tsuda Y
Faculty Of Pharmaceutical Sciences And High Technology Research Center Kobe Gakuin University
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岡本 成羽
Molecular Chemistry Laboratory Takeda Chemical Industries Ltd.
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