Determination of Covalently Bound Hypusine and Deoxyhypusine to Protein Using Submilligram of Protein Samples by HPLC
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概要
- 論文の詳細を見る
A sensitive and reliable method for the determination of hypusine and deoxyhypusine in eIF-5A protein, an initiation factor of protein synthesis, was developed. An advantage of this method is the use of N^ε-(5-aminopentyl) lysine, an analogue of deoxyhypusine, as an internal standard. The application made it possible to determine hypusine in less than a mg of protein samples from cultured HTC cells and rat organs. After acid hydrolysis of protein samples to which had been added the internal standard, the hydrolysates were fractionated by carboxymethyl cellulose column chromatography. Also, diamine fractions containing a few pmol of hypusine and deoxyhypusine were successfully analyzed by a reversed phase HPLC with a fluorescence detection of o-phthalaldehyde. The method was applied for the determination of hypusine and deoxyhypusine in drug-treated HTC cells and normal rat organs. The results from HTC cells were discussed based on the known effects of each drug on hypusine biosynthesis.
- 公益社団法人日本薬学会の論文
- 1996-01-15
著者
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Shirahata Akira
産業医科大学 医学部小児科学
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Shirahata Akira
Department Of Pediatrics School Of Medicine University Of Occupational And Environmental Health
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Shirahata A
Fac. Of Pharmaceutical Sciences Josai Univ.
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Shirahata A
聖マリアンナ医科大学 医学部医学統計学分野
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Shirahata Akira
Univ. Occupational And Environmental Health Kitakyushu Jpn
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SAMEJIMA Keijiro
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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BEPPU Takanobu
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
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Beppu T
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
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Samejima K
Faculty Of Pharmaceutical Sciences Josai University
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Samejima Keijiro
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
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鮫島 啓二郎
Faculty Of Pharmaceutical Sciences Josai University
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Shirahata Akira
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
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Beppu Takanobu
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
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Shirahata Akira
Lab. Of Cellular Physiology Dep. Of Clinical Dietetics And Human Nutrition Fac. Of Pharmaceutical Sc
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