Amine Modification of Digested Peptide at C-Terminal End during Protein Digestion by Protease(Biochemistry)
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概要
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We recently reported that C-terminal polyamine modification occurs when proteins are digested with trypsin in the presence of polyamine [Biochem. Biophys. Res. Commun., 356, 159-162 (2007)]. In the present study, the characteristics of this C-terminal modification in the presence of protease and amine were investigated. When hemoglobin (HB) was digested with trypsin in the presence of N-(2-pyridyl)-1, 4-diaminobutane (Py4), formation of the modified peptide was dependent on time and on HB or Py4 concentration. When synthetic peptide was treated with trypsin in the presence of Py4, ca. 0.1% of the peptide was modified with Py4. When HB or cytochrome C was treated with a range of serine proteases in the presence of various amines (Py4, N-(2-pyridyl)-1, 3-diaminopropane, tranexamic acid, isonicotinic acid hydrazide and ampicillin), the modified peptide was detected in all cases tested, thus suggesting that amine modification widely accompanies digestion by proteases.
- 2007-10-01
著者
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IKEGUCHI Yoshihiko
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo
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Shirahata Akira
Department Of Pediatrics School Of Medicine University Of Occupational And Environmental Health
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Takao Koichi
Department Of Human Nutrition And Clinical Dietetics Faculty Of Pharmaceutical Sciences Josai Univer
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Takao Koichi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
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Shirahata Akira
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Josai University:department Of Human N
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Shirahata Akira
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
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ITO Toshiyuki
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Josai University
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SUGITA Yoshiyuki
Department of Human Nutrition and Clinical Dietetics, Faculty of Pharmaceutical Sciences, Josai Univ
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Ikeguchi Yoshihiko
Department Of Analytical Chemistry Faculty Of Pharmaceutical Science Science University Of Tokyo
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Sugita Yoshiyuki
Department Of Human Nutrition And Clinical Dietetics Faculty Of Pharmaceutical Sciences Josai Univer
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Ito Toshiyuki
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Josai University
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Sugita Yoshiaki
Department of Human Nutrition and Clinical Dietetics, Faculty of Pharmaceutical Sciences, Josai University
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