Arginyl-tRNA-Protein Transferase Activities in Crude Supernatants of Rat Tissues
スポンサーリンク
概要
- 論文の詳細を見る
A fluorescent HPLC method for the assay of arginyl-tRNA-protein transferase (R-Transferase) activity was applied to obtain quantitative data of the enzyme activity in rat tissues for the first time. In this assay, the major problem was a significant hydrolysis of the substrate, N-aspartyl-N'-dansylamido-1,4-butanediamine, and the product, N-arginylaspartyl-N'-dansylamido-1,4-butanediamine (ArgAsp(4)DNA) by aminopeptidases in crude samples such as 105000 g supernatants (105S) of tissue homogenates. As bestatin inhibited the hydrolysis of ArgAsp(4)DNA, a standard-addition method in the presence of bestatin, using a partially purified R-Transferase preparation from hog kidney as a standard, made it possible to measure directly R-Transferase activities in 105S with a short incubation time and sufficient reliability. It was found by the established method that of 14 tissues examined, stomach was rich in the R-Transferase activity with the highest specific activity, suggesting a target tissue for the future studies on R-Transferase to elucidate its physiological significance.
- 公益社団法人日本薬学会の論文
- 1999-09-15
著者
-
Takao Koichi
Department Of Human Nutrition And Clinical Dietetics Faculty Of Pharmaceutical Sciences Josai Univer
-
Takao Koichi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
-
SAMEJIMA Keijiro
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University
-
Samejima Keijiro
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Josai University
関連論文
- Assay of N1-Acetylpolyamine Oxidase Activity with N1,N11-Didansylnorspermine as the Substrate by Ion-Pair Reversed Phase High Performance Liquid Chromatography
- Inhibition of Putrescine Aminopropyltransferase Influences Rat Liver Regeneration(Analytical Biochemistry)
- Synthetic Decarboxylated S-Adenosyl-L-methionine as a Substrate for Aminopropyl Transferases
- Measurements of Macromolecule-Bound and Ultra-Filtrable Polyamines in Rat Liver Homogenized without Buffer
- Primary Structure of Rat Spermidine Synthase : An Example of Refining the cDNA-Derived Amino Acid Sequence
- Determination of Covalently Bound Hypusine and Deoxyhypusine to Protein Using Submilligram of Protein Samples by HPLC
- Specific Depletion of Spermidine and Spermine in HTC Cells Treated with Inhibitors of Aminopropyltransferases^1
- Antagonistic Effect of N - (3 - Aminopropyl)cyclohexylamine on Neurotrophic Action of Spermine in Primary Cultured Rat Hippocampal and Cerebellar Neurons
- Structural Requirement for Neurotrophic Activity of Spermine in Cultured Rat Hippocampal Neurons
- Mass Spectrometric Separation and Determination of N^1,N^-Diacetylspermine in the Urine of Cancer Patients
- Enhancement of Gastrointestinal Absorption of Ovalbumin Caused by Spermine Induces an Increase in Plasma Histamine Levels in Mice Sensitized to Ovalbumin
- Amine Modification of Digested Peptide at C-Terminal End during Protein Digestion by Protease(Biochemistry)
- Arginyl-tRNA-Protein Transferase Activities in Crude Supernatants of Rat Tissues
- ACTIVE SITE OF AMINOPROPYLTRANSFERASES AND DEVELOPMENT OF INHIBITORS
- Pentamines as Substrate for Human Spermine Oxidase