ポリアミンの調節を目指した方法論の開発
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概要
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This brief article is dedicated to the late Professor Morizo Ishidate, and concerned in a methodology developed by the author and collaborators, aiming at the regulation of polyamine, especially, the regulation of spermidine synthase. The content is separated in 3 sections. The first section on the development of analytical methods, contains seven items, e.g. fluorometric determination of polyamines by fluorescamine, analysis of naturally occurring polyamines by GC and GC-MS, etc. The second section on the syntheses of needed compounds, contains three items, e.g. syntheses of decarboxylated S-adenosylmethionine which is a substrate for spermidine synthase and its related compounds, syntheses of ^15N-enriched polyamines applicable to the preparation of various polyamines, etc. The last section on the studies of aminopropyl transferases, contains three items, e.g. purification of spermidine synthase from mammalian tissues using ATPA-Sepharose, a novel affinity carrier, newly developed inhibitors for the enzymes, etc.
- 1997-11-01
著者
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鮫島 啓二郎
城西大学薬学部
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Samejima K
Faculty Of Pharmaceutical Sciences Josai University
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鮫島 啓二郎
Faculty Of Pharmaceutical Sciences Josai University
関連論文
- Formation of Spermidine or Norspermidine from Synthetic Diacetylpolyamines by Acetylpolyamine Oxidase in Cultured Cells(Biochemistry)
- A New Ultra-micro Microbioassay
- Simultaneous Determination of Endogenous and Orally Administered 15N-Labeled Polyamines in Rat Organs
- Syntheses of [13C, 15N]-Labeled Polyamines
- Fate of Orally Administered 15N-Labeled Polyamines in Rats Bearing Solid Tumors
- Measurements of Macromolecule-Bound and Ultra-FiltrablePolyamines in Rat Liver Homogenized without Buffer
- Preparation and Usefulness of Some Fluorogenic Substrates for Assay of Arginyl-tRNA-Protein Transferase byHPLC
- Syntheses of [5,8-^C_2]- and [1,12-C_2]Spermine Using Potassium [^C]Cyanide as the ^C Source
- Synthetic Decarboxylated S-Adenosyl-L-methionine as a Substrate for Aminopropyl Transferases
- Mass Spectrometric Separation and Determination of N1,N12-Diacetylspermine in the Urine of Cancer Patients
- A Gas Chromatographic Determination Method of 5-Chloro-7-iodo-8-quinolinol and Its Conjugates in Biological Fluids
- Synthesis of 5-Chloro-7-iodo-8-quinolinol Sulfate
- Bifidus Factors in Carrot. I. Purification and Some Properties
- Inhibition of Putrescine Aminopropyltransferase Influences Rat Liver Regeneration(Analytical Biochemistry)
- Control of Spermidine and Spermine Levels in Rat Tissues by trans-4-Methylcyclohexylamine, a Spermidine-Synthase Inhibitor(Analytical Biochemistry)
- Mammalian Spermidine Synthase : Identification of Cysteine Residues and Investigation of the Putrescine Binding Site(Analytical Biochemistry)
- Synthetic Decarboxylated S-Adenosyl-L-methionine as a Substrate for Aminopropyl Transferases
- Fate of Orally Administered ^15N-LabeIed Polyamines in Rats Bearing Solid Tumors
- Measurements of Macromolecule-Bound and Ultra-Filtrable Polyamines in Rat Liver Homogenized without Buffer
- Primary Structure of Rat Spermidine Synthase : An Example of Refining the cDNA-Derived Amino Acid Sequence
- Determination of Covalently Bound Hypusine and Deoxyhypusine to Protein Using Submilligram of Protein Samples by HPLC
- Specific Depletion of Spermidine and Spermine in HTC Cells Treated with Inhibitors of Aminopropyltransferases^1
- Enzymatic Aminopropylation of Certain Secondary Amines
- A Simpler Synthesis of D-Pantothenic Acid 4'-Phosphate
- Primary Structure of Rat Spermidine Synthase: AnExample of Refining the cDNA-Derived Amino AcidSequence
- Antagonistic Effect of N - (3 - Aminopropyl)cyclohexylamine on Neurotrophic Action of Spermine in Primary Cultured Rat Hippocampal and Cerebellar Neurons
- Structural Requirement for Neurotrophic Activity of Spermine in Cultured Rat Hippocampal Neurons
- Mass Spectrometric Separation and Determination of N^1,N^-Diacetylspermine in the Urine of Cancer Patients
- スペルミジン過剰蓄積細胞における核成分の変化
- Occurrence of aminopropylhomospermidine as themajor cellular polyamine in a halophilic, phototrophicalpha proteobacterium, Rhodothalassium salexigens
- Occurrence of tertiary branched tetraamines in two aquaticplants
- IDENTIFICATION OF N^4-(2-PROPENAL) SPERMIDINE AS A MAJOR REACTION PRODUCT OF MALONDIALDEHYDE AND SPERMIDINE
- Syntheses of Tertiary Tetraamines and Quaternary Pentaamines with Three and Four Methylene Chain Units
- Analayses of Variously ^N-Enriched Spermidines during Growth of Candida coidinii Cultured with ^N-Enriched Ammonium Sulfate and α-^N-Enriched L-Ornithine
- Syntheses of a Series of Linear Pentaamines with Three and Four Methylene Chain Intervals
- Studies on Inhibition of Enzymatic Arginyltransfer Reaction
- ポリアミンの調節と癌
- Arginyl-tRNA-Protein Transferase Activities in CrudeSupernatants of Rat Tissues
- ポリアミンの調節を目指した方法論の開発
- アスパラギン酸アナログによるアルギニルトランスフェラーゼの阻害効果の検討
- Metabolism of 4-Dimethylaminoazobenzene and Related Compounds. III. Metabolites of 4-Dimethylaminoazobenzene in Rat Bile and Influence of DAB Feeding on their Amounts
- A Probable Structure of the Bifidus Factor in Carrot Root
- TRANS-4-METHYLCYCLOHEXYLAMINE, A POTENT NEW INHIBITOR OF SPERMIDINE SYNTHASE
- Metabolites of p-Dimethylaminoazobenzene in Rat Bile
- MICROBIOLOGICAL ULTRAMICRO DETERMINATION OF PANTOTHENIC ACID, BIOTIN, AND NICOTINIC ACID WITH AMPLIFIED CULTIVATION
- Metabolism of 4-Dimethylaminoazobenzene and Related Compounds. IV. Metabolites of o-Aminoazotoluene in Rat Bile.