Analayses of Variously ^<15>N-Enriched Spermidines during Growth of Candida coidinii Cultured with ^<15>N-Enriched Ammonium Sulfate and α-^<15>N-Enriched L-Ornithine

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The analytical method for various ^<15>N-enriched spermidines was first applied for studies on polyamine metabolism of the yeast, Candida boidinii CBS 5777. Analyses were carried out during growth of the yeast in two defined media containing ^<15>N-enriched ammonium sulfate as a sole nitrogen source, and newly prepared α-^<15>N-enriched L-ornithine with ammonium sulfate as nitrogen sources. Observation in the former culture indicated that the ammonia pool was negligible, and the putrescine pool was larger than the decarboxylated S-adenosylmethionine pool in this organism.The latter culture suggested that α, δ-nitrogens of ornithine behaved equally throughout polyamine metabolism.
公益社団法人日本薬学会の論文
1990-11-25