Formation of Spermidine or Norspermidine from Synthetic Diacetylpolyamines by Acetylpolyamine Oxidase in Cultured Cells(Biochemistry)
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概要
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Prodrugs that can readily release polyamine into cells without the problem of generating cytotoxic compound by serum amine oxidase would be extremely useful for elucidation of polyamine function. As linear polyamines with acetamide groups on both sides are thought to be stable in the presence of serum amine oxidase and produce polyamines by the catalytic reaction of acetylpolyamine oxidase (PAO), a series of diacetyltetraamines, diacetylpentaamines and diacetylhexaamines was prepared as prodrugs and tested for substrate activity against PAO, partially purified from rat liver. Of the compounds, N^1, N^<15>-diacetyl-1, 15-diamino-4, 8, 12-triazapentadecane (DA3333) and N^1, N^<16>-diacetyl-1, 16-diamino-4, 8, 13-triazahexadecane (DA3343) were found to be stable in culture medium containing newborn bovine serum, and to produce reasonable amounts of norspermidine and spermidine, respectively. DA3333 and DA3343 were then applied to 1-aminooxy-3-aminopropane (AOAP)-treated HTC cells with depleted putrescine and spermidine, and arrested growth. Cell growth recovered with DA3333 and DA3343, but growth rate was reduced in cells with added DA3333 compared with growth rates in cells with added DA3343 and control cells untreated with AOAP. Significant amounts of norspermidine and spermidine were found in cells with added DA3333 and DA3343, respectively. These results show the potential use of diacetylpolyamines in introducing polyamines into cells.
- 2007-12-01
著者
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TAKAO Koichi
Faculty of Pharmaceutical Sciences, Josai University
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OZAWA Tomohiro
Faculty of Pharmaceutical Sciences, Josai University
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SHIBATA Satoko
Faculty of Pharmaceutical Sciences, Josai University
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WADA Makiko
Faculty of Pharmaceutical Sciences, Josai University
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SUGITA Yoshiaki
Faculty of Pharmaceutical Sciences, Josai University
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SHIRAHATA Akira
Faculty of Pharmaceutical Sciences, Josai University
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SAMEJIMA Keijiro
Faculty of Pharmaceutical Sciences, Josai University
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Shirahata Akira
産業医科大学 医学部小児科学
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Shirahata A
Fac. Of Pharmaceutical Sciences Josai Univ.
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Shirahata A
聖マリアンナ医科大学 医学部医学統計学分野
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Shirahata Akira
Univ. Occupational And Environmental Health Kitakyushu Jpn
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Shirahata Akira
Faculty Of Pharmaceutical Science Josai University
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Wada Makiko
Fac. Of Pharmaceutical Sciences Josai Univ.
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Wada Makiko
Faculty Of Pharmaceutical Sciences Josai University
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Takao Koichi
Faculty Of Pharmaceutical Sciences Josai University
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Ozawa Tomohiro
Faculty Of Pharmaceutical Sciences Josai University
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Shibata Satoko
Faculty Of Pharmaceutical Sciences Josai University
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Sugita Yoshiaki
Faculty Of Pharmaceutical Sciences Josai University
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Samejima Keijiro
Faculty Of Pharmaceutical Sciences Josai University
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Samejima K
Faculty Of Pharmaceutical Sciences Josai University
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Takao K
Faculty Of Pharmaceutical Sciences Josai University
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鮫島 啓二郎
Faculty Of Pharmaceutical Sciences Josai University
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Shirahata Akira
Lab. Of Cellular Physiology Dep. Of Clinical Dietetics And Human Nutrition Fac. Of Pharmaceutical Sc
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