High Yield Selective Induction of Uterine Endometrial Adenocarcinomas in CD-1 Mice by N-Ethyl-N'-nitro-N-nitrosoguanidine Combined with 17β-Estradiol
スポンサーリンク
概要
- 論文の詳細を見る
In order to assess a combined carcinogen and hormone protocol for selective induction of uterine adenocarcinomas in mice, 79 illumination-induced persistent estrous CD-1 mice, divided into four groups, were treated with N-ethyl-N-nitro-N-nitrosoguanidine (ENNG) and/or 17β-estradiol (E2). Groups 1 and 3 were given a single intra-uterine administration of polyethylene glycol (PEG) at 10 weeks of age, while Groups 2 and 4 received ENNG (12.5 mg/kg), dissolved in PEG. Mice of Groups 3 and 4 were also implanted with E2 pellets s.c. one week earlier and the pellets were once renewed after 8 weeks. At 15 weeks after the ENNG treatment, the mice were killed for histopathological and endocrinological examination. All groups demonstrated endometrial proliferative lesions, although no severe hyperplasias or adenocarcinomas were found in the control group (Group 1). The incidences of adenocarcinomas in Groups 2, 3, and 4 were 6 (1/19), 25 (5/20), and 55% (11/20), respectively, those for Groups 3 and 4 being significant as compared to the lack in Group 1. The E2: progesterone (E2: P) ratios in Groups 3 and 4 were also significantly increased. These results indicate that an increased E2: P ratio is important for endometrial adenocarcinoma development in CD-1 mice, acting with or without chemical carcinogen initiation to cause uterine cancer development. The relatively short duration, and specifically high yield mean that the present protocol has advantages for two-stage uterine carcinogenicity studies in mice.
- 日本毒性病理学会の論文
- 1999-06-01
著者
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MAEKAWA AKIHIKO
Department of Pathology, Sasaki Institute
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YOSHIDA Midori
Department of Pathology, Sasaki Institute
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TAKAHASHI Masakazu
Department of Pathology, Sasaki Institute
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Iizuka Seiichi
New Drug Discovery Laboratory Tsumura & Co.
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Yoshida Midori
Div. Of Pathology National Inst. Of Health Sciences
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Yoshida Midori
Department Of Pathology Sasaki Institute
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KATSUDA Shin-ichi
Department of Biological Safety Research, Tama laboratory, Japan Food Research Laboratories
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Maekawa A
Chemical Management Center National Inst. Of Technol. And Evaluation
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Maekawa Akihiko
Chemical Management Center National Institute Of Technology And Evaluation
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Maekawa Akihiko
Department Of Pathology Nagoya City University Medical School
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SHIRAKI Katsuhisa
Department of Environment and Mutation, Research Institute for Radiation Biology and Medicine, Hiros
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NISHIMURA SUSUMU
Department of Internal Medicine, Sapporo Shirakabadai Hospital
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Maekawa Akihiko
Safety Assessment Division Chemical Management Center National Institute Of Technology And Evaluatio
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Katashima S
Sasaki Inst. Tokyo Jpn
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ANDO Jin
Department of Pathology, Sasaki Instiute
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KUDOH Kayoko
Department of Pathology, Sasaki Institute
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Kudoh Kayoko
Department Of Pathology Sasaki Institute
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Ando-lu Jin
Department Of Pathology Sasaki Instiute
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Takahashi Masakazu
Division Of Pathology National Institute Of Health Sciences
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Takahashi Masakazu
Department Of Microbiology Niigata College Of Pharmacy
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Katsuda Shin-ichi
Department Of Biological Safety Research Tama Laboratory Japan Food Research Laboratories
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Takahashi Miwa
National Institute of Health Sciences
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Shiraki Katsuhisa
Department Of Cellular Biology Research Institute For Radiation Biology And Medicine Hiroshima Unive
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Takahashi Masakazu
Department Of Pathology Sasaki Institute Sasaki Foundation
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Nishimura Susumu
Department Of Pathology Sasaki Institute
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Takahashi Masakazu
National Institute Of Health Sciences
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Takahashi Masakazu
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Takahashi Masakazu
Department Of Bioscience Faculty Of Biotechnology Fukui Prefectural University
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Ando Jin
Department of Organ Regulatory Surgery, Fukushima Medical University, School of Medicine
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