Dielectrophoresis Conditions for Pearl Chain Formation and Effect of Pulse Field Strength on Protoplast Breakdown of Hericium erinaceum
スポンサーリンク
概要
- 論文の詳細を見る
The fusion method using PEG as a fusogenic agent has a number of disadvantages in comparison with the newly developed electrical fusion method. However, there has been little application yet of this electrical fusion method to the fusion of protoplasts of mushrooms. In this report, we describe the pearl chain formation and effect of pulse field strength on protoplast breakdown of protoplasts prepared from Hericium erinaceum. The protoplasts density favorable for pearl chain formation was about 10^8protoplasts/ml, and the yield of single pairs was maximum (30%) after 60sec dielectrophoresis at 100V/cm and 1MHz. The field intensity required to decompose half of the protoplast from H. erinaceum was 8kV/cm in a 0.7M mannitol solution.
- 武庫川女子大学の論文
著者
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NODA Hiroko
Department of Food Science and Nutrition, Mukogawa Women's University
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Noda H
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Ohsugi M
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Noda Hiroko
Department Of Food Science And Nutrition Mukogawa Women's University
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Fukuda Shoko
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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OHSUGI Masahiro
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's U
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Okamura Tokumitsu
Institute of Bioscience, Mukogawa Women's University
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Sohgawa Emi
Department of Food Science and Nutrition, School of Home Life and Environmental Science, Mukogawa Wo
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Okamura T
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Okamura Tokumitsu
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Ohsugi Masahiro
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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NODA HIROKO
Department of Biochemistry, School of Medicine Nagoya University
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OHSUGI Masahiro
Department of Domestic Science, Mukogawa Womens University
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