Novel FAD-Dependent Glucose Dehydrogenase for a Dioxygen-Insensitive Glucose Biosensor
スポンサーリンク
概要
- 論文の詳細を見る
A novel FAD-dependent glucose dehydrogenase (FAD-GDH) was found and its enzymatic property for glucose sensing was characterized. FAD-GDH oxidized glucose in the presence of some artificial electron acceptors, except for O2, and exhibited thermostability, high substrate specificity and a large Michaelis constant for glucose. FAD-GDH was applied to an amperometric glucose sensor with Fe(CN)63− as a soluble mediator. The use of a relatively high concentration of Fe(CN)63− resulted in a good linearity between the current response and the glucose concentration, taking into account a large Michaelis constant for Fe(CN)63−. The glucose sensor was completely insensitive to O2 and responded linearly to glucose up to 30 mM. Compared to glucose, the response to other saccharides was negligible. The sensor can be stored at room temperature in a desiccator for at least one month without any change in the response or activity.
- 社団法人 日本農芸化学会の論文
- 2006-03-23
著者
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KANO Kenji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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IKEDA Tokuji
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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Kano K
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kano Kenji
Kyoto Univ. Kyoto Jpn
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Kano Kenji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Ikeda Tokuji
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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TSUJIMURA Seiya
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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KOJIMA Shinki
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
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SATO Mika
Ikeda Food Research Co., Ltd.
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SANADA Hirokazu
Ikeda Food Research Co., Ltd.
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OMURA Hironori
Ikeda Food Research Co., Ltd.
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Sanada Hirokazu
Ikeda Food Research Co. Ltd.
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Tsujimura Seiya
Kyoto Univ. Kyoto Jpn
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Tsujimura Seiya
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Tsujimura S
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Kojima Shinki
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Omura Hironori
Ikeda Food Research Co. Ltd.
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Kano Kenji
Division Of Applied Life Science Graduate School Of Agriculture Kyoto University
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Sato Mika
Ikeda Food Research Co. Ltd.
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TSUJIMURA Seiya
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University
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