Glucose Metabolism of Lactic Acid Bacteria Changed by Quinone-mediated Extracellular Electron Transfer(Microbiology & Fermentation Technology)

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It can be expected that extracellular electron transfer to regenerate NAD^+ changes the glucose metabolism of the homofermentative lactic acid bacteria. In this work, the glucose metabolism of Lactobacillus plantarum and Lactococcus lactis was examined in resting cells with 2-amino-3-carboxy-1, 4-naphthoquinone (ACNQ) as the electron transfer mediator and ferricyanide (Fe (CN)_6^<3->) as the extracellular electron acceptor. NADH in the cells was oxidized by ACNQ with the aid of diaphorase, and the reduced ACNQ was reoxidized with Fe (CN)_6^<3->. The extracellular electron transfer system promoted the generation of pyruvate, acetate, and acetoin from glucose, and restricted lactate production. Diaphorase activity increased when cultivation was aerobic, and this increased the concentrations of pyruvate, acetate, and acetoin relative to the concentration of lactate to increase in the presence of ACNQ and Fe (CN)_6^<3->.
社団法人日本農芸化学会の論文
2002-10-23