Glycosylation of the Alpha and Beta Tubulin by Sialyloligosaccharides(Biochemistry)
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概要
- 論文の詳細を見る
To examine whether α and β tubulin are glycoproteins, we used a pyridylamino labeling method and a monoclonal antibody, SG3-1, raised against NeuAcα2-3Gal structure. Alpha and β tubulin from both pig brain and HeLa cells were positive for the SG3-1 antibody by immunoblot assay. Sialidase treatment reduced the reactivity of the SG3-1 antibody to α and β tubulin molecules. N-linked oligosaccharide analysis also showed that α and β tubulin are glycosylated. Moreover, immunofluorescence analysis showed that the filamentous structure recognized by the SG3-1 antibody was overlapped with microtubules, especially in the vicinity of the nucleus. These results indicate that α and β tubulin are glycosylated with sialyloligosaccharides.
- 社団法人日本動物学会の論文
- 2003-06-25
著者
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HOSOYA Hiroshi
Department of Biological Science, Graduate School of Science,Hiroshima University
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Hino Mizuki
Department of Anatomy, Gunma University Graduate School of Medicine
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Hino Mizuki
広島大学 理研究
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Hino Mizuki
Department Of Biological Science Graduate School Of Science Hiroshima University:(present Address)de
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Hino Mizuki
Department Of Biological Science Graduate School Of Science Hiroshima University
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NAGAI Yoshitaka
Mitsubishi Kasei Institute of Life Science
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Kijima-Suda Isao
Laboratory of Racing Chemistry
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Hosoya Hiroshi
Department Of Biological Science Graduate School Of Science Hiroshima University
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Hosoya Hiroshi
Department Of Biological Science Faculty Of Science Hiroshima University
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Nagai Yoshitaka
Mitsubishi Kasei Institute Of Life Sciences
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