Synthesis and Characterization of a Carbene-Generating Biotinylated Lactosylceramide Analog as a Novel Chromogenic Photoprobe for GM_3 Synthase

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概要

• 論文の詳細を見る

• A new biotinylated lactose derivative bearing a nitro-substituted chromogenic diazirine was synthesized. The biotinyl group within the structure enabled the performance of a convenient assay of GM_3 synthase based on avidin-biotin technology, and the K_m values of this biotinylated photoprobe were determined as 40 and 47 μM using bovine brain and rat liver Golgi as enzyme sourced, respectively. Furthermore, the sialylation of lactosylceramide, a natural acceptor substrate for GM_3 synthase, was completitively inhibited by this synthetic analog. The reagent could be a useful chromogenic photoprobe for GM_3 synthase.

• 社団法人日本薬学会の論文
• 1996-05-15

著者

• HIDARI Kazuya

  Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Core Research

• Hidari Kazuya

  Department Of Biochemical Cell Research The Tokyo Metropolitan Institute Of Medical Science

• HATANAKA Yasumaru

  Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University

• HASHIMOTO Makoto

  Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University

• SANAI Yutaka

  Department of Biochemical Cell Research, The Tokyo Metropolitan Institute of Medical Science

• TEZUKA Yasuhiro

  Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University

• NAGAI Yoshitaka

  Mitsubishi Kasei Institute of Life Science

• KANAOKA Yuichi

  Toyama Womens College

• Sanai Y

  Tokyo Metropolitan Inst. Medical Sci.

• Sanai Yutaka

  Department Of Biochemical Cell Research

• Tezuka Yasuhiro

  Research Institute For Wakan-yaku (oriental Medicines) Toyama Medical And Pharmaceutical University

• Nagai Y

  Mitsubishi Kasei Institute Of Life Science

• Hatanaka Yasumaru

  University Of Toyama

• Hatanaka Yasumaru

  Research Institute For Wakan-yaku (oriental Medicines) Toyama Medical And Pharmaceutical University

• Hatanaka Y

  University Of Toyama

• Hashimoto M

  United Graduate School Of Agricultural Science Tokyo University Of Agriculture And Technology

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