The Effect of Kampo Formulae on Bone Resorption in Vitro and in Vivo. I. Active Constituents of Tsu-Kan-gan
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概要
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Four water extracts of Kampo formulae (Yi-kkan-sen, Dai-ho-in-gan, Ni-chi-gan, Tsu-kan-gan) were screened for their inhibitory activities on bone resorption induced by parathyroid hormone (PTH) in organ culture using neonatal mouse parietal bones. Among the Kampo formulae, Tsu-kan-gan (TKG) showed the most potent inhibitory activity. We further fractionated the TKG water extract by monitoring the inhibitory activity on bone resorption stimulated by PTH in virto. The MeOH fraction of the water extract inhibited PTH-stimulated bone resorption, and its inhibitory activity was more potent than those of other fractions. The MeOH fraction was then subjected to Sephadex LH-20 column chromatography to give fractions I, II and III, which were examined for bone resorption activity. Fraction I inhibited PTH-stimulated bone resorption, and its inhibitory activity was more potent than those of the other fractions. Upon oral administration of the three fractions (100 mg/kg/d) to ovariectomized (OVX) mice, fractions I and III prevented the decrease of bone mineral density (BMD) of the lumbar vertebra. Eleven compounds isolated from the MeOH fraction were examined for their inhibitory effect on PTH-stimulated bone resorption. Among them, berberine (1), syringin (3), limonin (4) and mangiferin (10) showed a significant inhibitory effect on bone resorption. In the formation assay of osteoclast-like cells, these compounds decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (MNCs). The inhibitory effect of TKG on bone resorption may be at least partly due to the inhibitory action of these compounds.
- 社団法人日本薬学会の論文
- 1998-12-15
著者
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KALAUNI Surya
Institute of Natural Medicine, University of Toyama
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Seto Hikaru
Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Seto Hikaru
富山医科薬科大学和漢薬研究所
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Kadota S
Institute Of Natural Medicine University Of Toyama
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TEZUKA Yasuhiro
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University
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Namba Ttuneo
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Namba Tsuneo
富山医科薬科大学 生薬
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Namba Tsuneo
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Nagaoka Takema
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Nagaoka Takema
富山医科薬科大学和漢薬研究所
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MIYAHARA Tatsuro
Faculty of Medicine, University of Toyama
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Tezuka Yasuhiro
Research Institute For Wakan-yaku (oriental Medicines) Toyama Medical And Pharmaceutical University
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Miyahara T
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Miyahara Tatsuro
Faculty Of Medicine University Of Toyama
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LI Huiying
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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NEMOTO Nobuo
Faculty of Pharmaceutical Science, Toyama Medical and Pharmaceutical University
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TONAMI Syuichi
Faculty of Medicine, Toyama Medical and Pharmaceutical University
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LI Huiying
Research Institute of Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceu
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KADOTA Shigetoshi
Research Institute of Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceu
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Kadota Shigetoshi
Research Institute For Wakan-yaku (oriental Medicines) Toyama Medical And Pharmaceutical University
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Li Huiying
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Nemoto N
Toyama Medical & Pharmaceutical Univ. Toyama Jpn
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Nemoto Nobuo
Faculty Of Medicine And Department Of Toxicology Faculty Of Pharmaceutical Sciences Toyama Medical
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Tada Takahiro
Research & Development Department Mikimoto Pharmaceutical Co. Ltd.
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Tonami S
Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Tonami Syuichi
Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Namba Tsuneo
Research Institute For Wakan-yaku (traditional Sino-japanese Medicine) Toyama Medical And Pharmaceut
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Namba Tsuneo
Research Institute For Wakan-yaku (oriental Medicine) Toyama Medical And Pharmaceutical University
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門田 重利
Institute of Natural Medicine, University of Toyama
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