The Effect of Kampo Formulae on Bone Resorption in Vitro and in Vivo. II. Detailed Study of Berberine
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概要
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We previously isolated berberine from aqueous extracts of tsu-kan-gan, a Kampo formula used for the treatment of osteoporosis. Berberine caused an inhibitory effect on parathyroid hormone (PTH)-stimulated bone resorption in neonatal mouse bone. In this report we describe the inhibitory effect of berberine on the formation of osteoclast-like multinucleated cells (OCLs) in the co-culture of mouse osteoblastic cells and bone marrow cells in the presence of 1α, 25-dihydroxyvitamin D_3 [1α, 25(OH)_2D_3], PTH and interleukin-1α (IL-1α). Berberine dosedependently inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive OCLs induced by 1α, 25 (OH)_2D_3,PTH and IL-1α. We prepared OCLa in the co-culture of osteoblastic cells and bone marrow cells. The effect of berberine on pit formation by OCLs was examined using dentin slices. As OCLs are terminally differentiated multinucleated calls, the survival of OCLs affects the bone-resorbing activity of OCLs. This prompted us to count the number of TRAP-positive OCLs on the slices. Berberine dose-dependently inhibited pit formation and caused a decrease in the number of TRAP-positive OCLs. Calctinin (CT) inhibited pit formation without affecting the number of OCLs. Berberine accelerated the cell death in OCLs cultivated on a culture plate, but CT did not affect the cell death of OCLs. This suggestes that the decrease in the number of OCLs on dentin slices may be due to apoptotic cell death in OCLs. In fact, Hoechst 33258 staining revealed that the treatment of OCLs with berberine resulted in condensed nuclei and a decrease in cell size. Oral administration of the berberine (30 and 50 mg/kg/d) to ovariectomized ras prevented a decrease in bone mineral density (BMD) of the lumber vertebra without affecting the weight of the uterus and plasma concentration of estradiol. These results suggested that berberine prevented a decrease in BMD in vivo by inhibiting osteoclastic bone resorption.
- 社団法人日本薬学会の論文
- 1999-04-15
著者
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KADOTA Shigetoshi
Institute of Natural Medicine, University of Toyama
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TEZUKA Yasuhiro
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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KALAUNI Surya
Institute of Natural Medicine, University of Toyama
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Seto Hikaru
Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Seto Hikaru
富山医科薬科大学和漢薬研究所
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Kadota S
Institute Of Natural Medicine University Of Toyama
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Namba Ttuneo
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Namba Tsuneo
富山医科薬科大学 生薬
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Nagaoka Takema
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Nagaoka Takema
富山医科薬科大学和漢薬研究所
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Watanabe Makoto
Faculty of Health Science, Kobe University School of Medicine
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MIYAHARA Tatsuro
Faculty of Medicine, University of Toyama
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Tezuka Yasuhiro
Institute Of Natural Medicine Univeristy Of Toyama
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Miyahara T
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Miyahara Tatsuro
Faculty Of Medicine University Of Toyama
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Namba Tsuneo
Institute Of Natural Medical Toyama Medicine And Pharmaceutical University
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LI Huiying
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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SUZUKI Takayuki
Faculty of Pharmaceutical Science, Toyama Medical and Pharmaceutical University
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DOWAKI Risa
Faculty of Pharmaceutical Science, Toyama Medical and Pharmaceutical University
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NEMOTO Nobuo
Faculty of Pharmaceutical Science, Toyama Medical and Pharmaceutical University
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TONAMI Syuichi
Faculty of Medicine, Toyama Medical and Pharmaceutical University
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Kadota Shigetoshi
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Li Huiying
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Dowaki Risa
Faculty Of Pharmaceutical Science Toyama Medical And Pharmaceutical University
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Nemoto N
Toyama Medical & Pharmaceutical Univ. Toyama Jpn
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Nemoto Nobuo
Faculty Of Medicine And Department Of Toxicology Faculty Of Pharmaceutical Sciences Toyama Medical
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Takamura Shuichi
Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Takamura S
Graduate School Of Engineering Nagoya University
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Tonami Syuichi
Faculty Of Medicine Toyama Medical And Pharmaceutical University
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Suzuki Takayuki
Faculty Of Pharmaceutical Science Toyama Medical And Pharmaceutical University
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Watanabe Makoto
Faculty Of Engineering Chiba University
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Watanabe Makoto
Faculty Of Pharmaceutical Science Toyama Medical And Pharmaceutical University
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門田 重利
Institute of Natural Medicine, University of Toyama
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