Novel Gene Encoding a Ca^<2+>-Binding Protein and under Hexokinase-dependent Sugar Regulation(Biochemistry & Molecular Biology)

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A cDNA encoding a predicted 15-kDa protein was earlier isolated from sugar-induced genes in rice embryos (Oryza sativa L.) by cDNA microarray analysis. Here we report that this cDNA encodes a novel Ca^<2+>-binding protein, named OsSUR1 (for Oryza sativa sugar-up-regulated-1). The recombinant OsSURl protein expressed in Escherichia coli had ^<45>Ca^<2+>-binding activity. Northern analysis showed that the OsSUR1 gene was expressed mainly in the internodes of mature plants and in embryos at an early stage of germination. Expression of the OsSUR1 gene was induced by sugars that could serve as substrates of hexokinase, but expression was not repressed by Ca^<2+> signaling inhibitors, calmodulin antagonists and inhibitors of protein kinase or protein phosphatase. These results suggested that Os-SUR1 gene expression was stimulated by a hexokinase-dependent pathway not mediated by Ca^<2+>.
社団法人日本農芸化学会の論文
2003-02-23