FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT
スポンサーリンク
概要
- 論文の詳細を見る
Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population(85.2%)was in good agreement with that for the tailless sperm(88.7%)determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication(r=0.94, P<0.01), or nitrobenzene(r=0.80, P<0.01). The results indicated the utility of the light scatter-histogram in flow cytometry as a simple and convenient procedure for the detection of tailless sperm induced by chemical compounds.
- 日本トキシコロジー学会の論文
- 2000-02-28
著者
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Yamamoto Toshio
食品農医薬品安全性評価センター
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Yamamoto Toshio
Biosafety Research Center Foods Drugs And Pesticides
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Yamamoto T
Showa Univ. Tokyo Jpn
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Matsui Takane
United Graduate School Of Veterinary Sciences Gifu Unicersity
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Yoneyama Mitsuru
Drug Development Laboratories Safety Evaluation Yoshitomi Pharmaceutical Industries Ltd.
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YAMAMOTO Toshinobu
Drug Development Laboratories, Safety Evaluation, Yoshitomi Pharmaceutical Industries, Ltd.
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IMANISHI Masanori
Drug Development Laboratories, Safety Evaluation, Yoshitomi Pharmaceutical Industries, Ltd.
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TAKEUCHI Masaki
Drug Development Laboratories, Safety Evaluation, Yoshitomi Pharmaceutical Industries, Ltd.
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Yamamoto T
Department Of Clinical Pharmacy School Of Pharmaceutical Sciences Showa University
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Takeuchi M
Safety Evaluation Drug Development Laboratories Pharmaceutical Research Division Yoshitomi Pharmaceu
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Tezuka M
Nihon Univ. Chiba Jpn
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Takeuchi Masaki
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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Yamamoto T
Inst. Soc. Techno‐innovation Agriculture Forestry And Fisheries (staff) Ibaraki Jpn
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Imanishi Masanori
Drug Development Laboratories Safety Evaluation Yoshitomi Pharmaceutical Industries Ltd.
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