EVALUATION OF RAT SPERM BY FLOW CYTOMETRY : SIMULTANEOUS ANALYSIS OF SPERM COUNT AND SPERM VIABILITY
スポンサーリンク
概要
- 論文の詳細を見る
In this study, we conducted a simultaneous analysis of sperm count and viability in rats by flow cytometry (FCM). Epididymal fluids were taken from the caudal epididymis of 12 to 13 week-old Sparague-Dawley rats. The fluids were weighed and mixed with Dulbecco's phosphate buffered saline (D-PBS). Propidium iodide, which can stain only dead sperm, was used to distinguish viable and dead sperm. The sperm count and viability analyzed by FCM were 1.28×10^6/mg and 78.0%, respectively. These values were consistent with the corresponding values (1.39×10^6/mg and 81.0%) that were directly determined microscopically in the fluids of the same sample. In addition, when the original mixture containing sperm was diluted two times and four times with D-PBS, or was diluted two times with D-PBS containing only killed sperm, the sperm count and viability determined by FCM also correlated well with the sperm count (r=0.96, P<0.01) and sperm motility (r=0.99, P<0.01) by direct microscopic observation, respectively. In conclusion, the present flow cytometric analysis would be practical for the simultaneous determination of sperm count and viability in rat epididymal fluids.
- 日本トキシコロジー学会の論文
著者
-
Yamamoto Toshio
食品農医薬品安全性評価センター
-
Yamamoto Toshio
Biosafety Research Center Foods Drugs And Pesticides
-
Yamamoto T
Showa Univ. Tokyo Jpn
-
Matsui Takane
United Graduate School Of Veterinary Sciences Gifu Unicersity
-
Yoneyama Mitsuru
Drug Development Laboratories Safety Evaluation Yoshitomi Pharmaceutical Industries Ltd.
-
YAMAMOTO Toshinobu
Drug Development Laboratories, Safety Evaluation, Yoshitomi Pharmaceutical Industries, Ltd.
-
IMANISHI Masanori
Drug Development Laboratories, Safety Evaluation, Yoshitomi Pharmaceutical Industries, Ltd.
-
YAMAMOTO Toshinobu
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industories, Ltd.
-
MORI Shigekazu
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industories, Ltd.
-
YONEYAMA Mitsuru
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industories, Ltd.
-
IMANISHI Masanori
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industories, Ltd.
-
TAKEUCHI Masaki
Yoshitomi Safety Evaluation Laboratory, Yoshitomi Pharmaceutical Industories, Ltd.
-
Yamamoto T
Department Of Clinical Pharmacy School Of Pharmaceutical Sciences Showa University
-
Mori Shigekazu
Yoshitomi Safety Evaluation Laboratory Yoshitomi Pharmaceutical Industories Ltd.
-
Takeuchi M
Safety Evaluation Drug Development Laboratories Pharmaceutical Research Division Yoshitomi Pharmaceu
-
Tezuka M
Nihon Univ. Chiba Jpn
-
Takeuchi Masaki
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
-
Yamamoto T
Inst. Soc. Techno‐innovation Agriculture Forestry And Fisheries (staff) Ibaraki Jpn
-
Imanishi Masanori
Drug Development Laboratories Safety Evaluation Yoshitomi Pharmaceutical Industries Ltd.
関連論文
- Sparse Distribution of Hepatocyte Growth Factor-producing Cells inside Hepatocellular Foci in Rats Treated with Hepatocarcinogens
- Enhancing Effects β-Estradiol 3-Benzoate but Not Methoxychlor on the Promotion/Progression Stage of Chemically-induced Mammary Carcinogenesis in Ovariectomized Rats
- EVALUATION OF HUMAN HEPATOCYTE CHIMERIC MICE AS A MODEL FOR TOXICOLOGICAL INVESTIGATION USING PANOMIC APPROACHES : EFFECT OF ACETAMINOPHEN ON THE EXPRESSION PROFILES OF PROTEINS AND ENDOGENOUS METABOLITES IN LIVER, PLASMA AND URINE
- INVESTIGATION OF PROTEOMIC BIOMARKERS IN IN VIVO HEPATOTOXICITY STUDY OF RAT LIVER : TOXICITY DIFFERENTIATION IN HEPATOTOXICANTS
- IN VIVO HEPATOTOXICITY STUDY OF RATS IN COMPARISON WITH IN VITRO HEPATOTOXICITY SCREENING SYSTEM
- Investigation of protein expression induced by hepatotoxicants in rat hepatocytes cultures and livers(Proceedings of the 32nd Annual Meeting)
- Comparison between in vitro- and in vivo-hepatotoxicity for the investigation of a hepatotoxicity screening system in primary cell cultures(Liver, Alimentary system, Proceedings of the 32nd Annual Meeting)
- IDENTIFICATION OF OXIDATIVE STRESS-RELATED PROTEINS FOR PREDICTIVE SCREENING OF HEPATOTOXICITY USING A PROTEOMIC APPROACH
- EFFECTS OF MALE REPRODUCTIVE TOXICANTS ON GENE EXPRESSION IN RAT TESTES
- PROTEIN EXPRESSION ANALYSIS OF RAT TESTES INDUCED TESTICULAR TOXICITY WITH SEVERAL REPRODUCTIVE TOXICANTS
- INVESTIGATION OF A HEPATOTOXICITY SCREENING SYSTEM IN PRIMARY CELL CULTURES : WHAT BIOMARKERS WOULD NEED TO BE ADDRESSED TO ESTIMATE TOXICITY IN CONVENTIONAL AND NEW APPROACHES?
- Possible enhancing effects of atrazine on growth of 7, 12-dimethylbenz(a) anthracene-induced mammary tumors in ovariectomized Sprague-Dawley rats
- Induction of pulmonary fibrosis by methotrexate treatment in mice lung in vivo and in vitro
- IMMUNOHISTOCHEMICAL STUDIES OF TSH-PRODUCING CELLS IN THE PITUITARY AND EXPRESSION OF GROWTH FACTORS IN THYROIDAL PROLIF-ERATIVE LESIONS IN RATS TREATED WITH THIOUREA AND EXCESS VITAMIN A
- Inhibition of MAP kinase activity moderates changes in expression and function of Cx32 but not claudin-1 during DNA synthesis in primary cultures of rat hepatocytes
- Regulation of the blood-biliary barrier : interaction between gap and tight junctions in hepatocytes
- P7-24 Investigation of the hepato-toxicity screening system in primary cell culture(IMMUNE AND ALLERGIC SYSTEM/KIDNEYS AND URINARY EXCRETION SYSTEM/CYTOTOXICITY/OTHERS-1)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 31st Annual Meeting)
- P6-46 Effect of reproductive toxicants on the protein expression in the rat testis(TOXICOGENOMICS)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 31st Annual Meeting)
- P6-45 Effect of reproductive toxicants on the gene expression in the testes of rats(TOXICOGENOMICS)(GENERAL SESSION BY POSTER PRESENTATION)(Proceedings of the 31st Annual Meeting)
- Point Mutations of the c-H-ras Gene in Spontaneous Pulmonary Tumors of Transgenic Mice Carrying the Human c-H-ras Gene
- FLOW CYTOMETRIC DETECTION AND ANALYSIS OF TAILLESS SPERM CAUSED BY SONICATION OR A CHEMICAL AGENT
- 22A-09-3 Flow cytometric analysis (FCM) of sperms taken from rats treated with nitrobenzene (NB).
- EVALUATION OF RAT SPERM BY FLOW CYTOMETRY : SIMULTANEOUS ANALYSIS OF SPERM COUNT AND SPERM VIABILITY
- COLLABORATIVE WORK TO DETERMINE AN OPTIMAL ADMINISTRATION PERIOD AND OPTIMAL PARAMETERS FOR DETECTION OF EFFECTS ON MALE FERTILITY IN RATS : MALE REPRODUCTIVE TOXICITY STUDY OF HALOPERIDOL (<SPECIAL ISSUE>TESTICULAR TOXICITY)
- Scattering Observables of NN Interaction in the SU_6 Quark Model
- Effects of Reproduction on Spontaneous Development of Endometrial Adenocarcinomas and Mammary Tumors in Donryu Rats
- Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes
- ORAL CHRONIC TOXICITY AND CARCINOGENICITY TEST OF POLYOXYETHYLENE(10)NONYLPHENYL ETHER (NP-10) IN FEMALE F344 RATS
- CARCINOGENICITY TEST OF POLYOXYETHYLENE(10)NONYLPHENYL ETHER (NP-10) IN FEMALE B6C3F_1 MICE
- ANALYSIS OF BACKGROUND PATHOLOGICAL LESIONS SEEN IN LONG-TERM TOXICITY TESTS (1). THE COMBINED HISTOPATHOLOGICAL CHANGES OBSERVED IN RATS OR MICE
- Size Distribution of Dew Droplets and Dew Formation Effect on Deposition Fluxes
- Controlling Factors of Weak Acid and Base Concentrations in Urban Dewwater : Comparison of Dew Chemistry with Rain and Fog Chemistry
- EMESIS-RELATED BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES INDUCED BY CISPLATIN IN THE FERRET
- B-20 ENTERIC 5-HT LEVELS AND KARYORRHEXIS INDUCED BY ANTINEOPLASTIC AGENTS AND COPPER SULFATE OF THE FERRET
- Establishment and Peculiarity of Ouabain-resistant THP-1 cells
- INITIAL METABOLISM OF GAMMA-HEXACHLOROCYCLOHEXANE (γ-HCH) BY RAT LIVER MICROSONES
- COMPARISON OF THE EFFECT OF AN EQUIMOLAR AND LOW DOSE OF FENITROTHION AND METHYLPARATHION ON THEIR OWN METABOLISM IN RAT LIVER
- PROTECTIVE EFFECTS OF DIISOPROPYL 1, 3-DITHIOL-2-YLIDENE MALONATE (NKK-105) ON LIVER INJURY BY CARBON TETRACHLORIDE (CCl_4)
- ALTERATIONS OF HEPATIC MITOCHONDRIAL MONOAMINE OXIDASE ACTIVITY OF RATS DURING 2-ACETYLAMINOFLUORENE FEEDING
- Methods for Imaging and Analyses of Intracellular Organelles Using Fluorescent and Luminescent Proteins
- Involvement of Adenosine A_2 Receptors in the Changes of Tissue Factor-Dependent Coagulant Activity Induced by Polymorphonuclear Leukocytes in Endothelial Cells
- Induction and Inhibition of Cytochrome P450 and Drug-Metabolizing Enzymes by Climbazole
- P12-08 Effect of 4-(4-Chlorobenzyl)pyridine on Rat Hepatic Microsomal Cytochrome P450 and Drug-metabolizing Enzymes In Vivo and In Vitro
- CLIMBAZOLE IS A NEW POTENT INDUCER OF RAT HEPATIC CYTOCHROME P450
- Changes of Tissue Factor-Dependent Coagulant Activity Mediated by Adhesion Between Polymorphonuclear Leukocytes and Endothelial Cells
- Effect of 4-(4-Chlorobenzyl)pyridine on Rat Hepatic Microsomal Cytochrome P450 and Drug-Metabolizing Enzymes in Vivo and in Vitro
- SEX-RELATED EFFECT OF HEMIN ON CYTOCHROME P450 AND DRUG-METABOLIZING ENZYMES IN RAT LIVER
- Antigenic characterization in ampiroxicam-induced photosensitivity using an in vivo model of contact hypersensitivity
- Toxicomarker Discovery for Hepatotoxicity using Proteome Analysis Technology (TOXICOGENOMICS AND TOXICOPROTEOMICS) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- COLLABORATIVE WORK TO EVALUATE TOXICITY ON MALE REPRODUCTIVE ORGANS BY REPEATED DOSE STUDIES IN RATS : 6)2-AND 4-WEEKS ADMINISTRATION STUDY OF HALOPERIDOL
- LARGE AMOUNT OF VITAMIN A HAS NO MAJOR EFFECTS ON THYROIDAL HORMONE SYNTHESIS IN TWO-STAGE RAT THYROID CARCINOGENESIS MODEL USING N-BIS(2-HYDROXYPROPYL)NITROSAMINE AND THIOUREA
- 21C-11-1 A study on mechanisms of the enhanced thyroidal tumorigenesis in rats by large amounts of vitamin A simultaneously given with thiourea : Effects on thyroidal hormone syntheses.
- Infected Papillary Fibroelastoma Attached to the Atrial Septum
- Isolation of a Tobacco cDNA Encoding Sar1 GTPase and Analysis of Its Dominant Mutations in Vesicular Traffic Using a Yeast Complementation System
- Nicorandil Infusion Accelerates ST-Segment Resolution in Patients with Acute Myocardial Infarction : Subanalysis in SMART Study
- Ribosomal protein L3 mediated the transport of digoxin in Xenopus laevis oocyte
- cDNA Cloning of Nuclear Localization Signal Binding Protein NBP60, a Rat Homologue of Lamin B Receptor, and Identification of Binding Sites of Human Lamin B Receptor for Nuclear Localization Signals and Chromatin^1
- Characterization of p92, Karyopherin β, Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes
- Preparation of Hyperbranched Aromatic Poly(ethersulfone)s Possessing Sulfonic Acid Terminal Groups for Polymer Electrolyte
- EFFECT OF 4-PHENYL-1, 3-DITHIA-2-THIOXO-CYCLOPENT-4-ENE ON LIVER INJURY INDUCED BY REPEATED EXPOSURE TO GALACTOSAMINE PLUS CARBON TETRACHLORIDE IN RATS
- EFFECT OF 1,3-DITHIA-2-THIOXO-CYCLOPENT-4-ENE AND ITS DERIVATIVES ON LIVER INJURY INDUCED BY CARBON TETRACHLORIDE AND OROTIC ACID IN RATS
- Does Different D-dimer Reduce the Use of Duplex Scanning to Rule out DVT in Patients with PE?
- Evaluation of fluence and pulse-duration on purpuric threshold using an extended pulse pulsed-dye laser in the treatment of port wine stains
- Expression and regulation of hepatic gap junctions by interleukin-1β (LIVER/DIGESTIVE SYSTEM) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Comparison of Capsular Morphology between two Different Surface Structures of Silicone Implants
- Influence of H_2O on the SiO_2 Growth by Parallel-Resonant RF Plasma Oxidation
- Enhanced Growth of Silicon Dioxide Films by Parallel-Resonant RF Plasmas
- The Case for a Shift in Pharmacists' Activities and Pharmacy Education : Based on Those in The English Speaking Western Countries
- Pharmaceutical Terms Reflecting the Change in Practice in Japan
- METHAMPHETAMINE-INDUCED BEHAVIORAL EFFECTS AND RELEASES OF BRAIN CATECHOLAMINES AND BRAIN CONCENTRATIONS OF METHAMPHETAMINE IN MICE
- EFFECTS OF CEPHEM ANTIBIOTICS ON RAT LIVER ALDEHYDE DEHYDROGENASES
- NADPH-DEPENDENT LIPID PEROXIDATION IN THE LIVER MICROSOME OF RATS FED A 3-METHYL-4-DIMETHYL-AMINOAZOBENZENE (3-Me-DAB)-CONTAINING DIET
- Pathological Features of Helicobacter pylori-Induced Gastritis in Mongolian Gerbils
- "C-3 Effect of the sperm of rats treated with Methacrylic Acid, 2,3-Epoxypropyl Ester.
- INHIBITION OF MONOAMINE OXIDASE BY 3-METHYL-4-DIMETHYLAMINO AZOBENZENE (3-Me-DAB) IN RAT LIVER MITOCHONDRIA
- Functional characterization and substrate specificity of a novel gene encoding zinc finger-like protein, ZfLp, in Xenopus laevis oocytes
- PRODUCTION OF TOLERANCE TO FENITROTHION I N MALE RATS
- Effect of Butylated Hydroxytoluene on Cell Population in Rat Hepatocytes
- Characteristic Formation of Edge Transport Barrier in the Compact Helical System
- First Observation of High Density Edge Transport Barrier Formation during Reheat Mode of Helical Plasma in CHS
- Characterization of p92, Karyopherin .BETA., Co-Purified with N-Acetylglucosamine-Bearing Nucleoporins from Rat Liver Nuclear Envelopes.