EFFECTS OF MALE REPRODUCTIVE TOXICANTS ON GENE EXPRESSION IN RAT TESTES
スポンサーリンク
概要
- 論文の詳細を見る
Predictive biomarkers of testicular toxicity are needed for an efficient development of drugs. The purpose of the present study was to obtain further insight into the toxicity mechanisms of various male reproductive toxicants and to detect genomic biomarkers for rapid screening of testicular toxicity. Four reproductive toxicants, 2, 5-hexanedione (Sertoli cells toxicant), ethylene glycol monomethyl ether (EGME; spermatocytes toxicant), cyclophosphamide (spermatogonia toxicant) and sulfasalazine, were orally administered to male rats once. Six hours after the single dosing, gene expression in the testes was monitored by cDNA microarray and real-time RT-PCR and the testes were histopathologically examined. No histopathological abnormality was detected except for slight degeneration of spermatocytes in the EGME-treated testes. cDNA microarray analysis revealed differential gene expression profiles, and it was possible based on the profiles to characterize the action of the compounds in the testes. Interestingly, 3 spermatogenesis-related genes - heat shock protein 70-2, insulin growth factor binding protein 3 and glutathione S transferase pi - were affected by all the compounds. The above changes of gene expression were detectable within a short period after the dosing prior to the appearance of obvious pathological changes. These data suggest that cDNA microarray is a useful technique for evaluation of primary testicular toxicity. Furthermore, we propose the above 3 spermatogenesis-related genes as potential biomarkers of testicular toxicity.
- 日本トキシコロジー学会の論文
- 2005-08-29
著者
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HORII Ikuo
Worldwide Safety Sciences, Nagoya Laboratories, Pfizer Global Research and Development, Pfizer Japan
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Yamamoto Toshinori
Drug Safety Research & Development Pfizer Global Research & Development Nagoya Laboratories
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Yamamoto T
Showa Univ. Tokyo Jpn
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Yamamoto Toshinori
Department Of Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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Mori C
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
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Mori Chisato
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
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Mori Chisato
千葉大学 医学研究院環境生命医学
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Horii Ikuo
Showa Univ. (department Of Biochemical Toxicology School Of Pharmaceutical Sciences)
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Horii I
Drug Safety Research And Development Pfizer Global Research And Development Nagoya Laboratories. Pfi
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Horii Ikuo
Pfizer Global Research & Development Nagoya Laboratories Pfizer Inc.
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YAMAMOTO Toshinori
Worldwide Safety Sciences, Pfizer Global Research & Development, Nagoya Laboratories, Pfizer Inc.
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KIKKAWA Rie
Worldwide Safety Sciences, Pfizer Global Research & Development, Nagoya Laboratories, Pfizer Inc.
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HAMADA Yoshimasa
Worldwide Safety Sciences, Pfizer Global Research and Development, Nagoya Laboratories, Pfizer Inc.
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FUKUSHIMA Tamio
Worldwide Safety Sciences, Global Research & Development, Nagoya Laboratories, Pfizer Inc.
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KOMIYAMA Masatoshi
Department of Bioenvironmental Medline, Chiba Univ.
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Mori Chisato
Department Of Anatomy Faculty Of Medicine Kyoto University
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Kikkawa Rie
Worldwide Safety Sciences Pfizer Global Research And Development Nagoya Laboratories Pfizer Inc.
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Kikkawa Rie
Worldwide Safety Sciences Global Research & Development Nagoya Laboratories Pfizer Inc.
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Komiyama M
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
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Komiyama Masatoshi
Department Of Bioenvironmental Medicine:center For Environment Health And Field Sciences Chiba Unive
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Komiyama Masatoshi
Department Of Anatomy And Cell Biology School Of Medicine Chiba Unversity
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Moria Chisato
Department Of Bioenvironmental Medicine:environmental Health Science Project For Future Generations
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Mori Chisato
Department Of Anatomy And Developmental Biology
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Yamada Hiroshi
Drug Safety Research & Development Pfizer Global Research & Development Nagoya Laboratories
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Fukushima Tamio
Drug Safety Research And Development Nagoya Laboratories Pfizer Japan Inc.
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Fukushima Tamio
Worldwide Safety Sciences Pfizer Global Research & Development Nagoya Laboratories Pfizer Inc.
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Fukushima Tamio
Pfizer Global Research & Development Toxicology Nagoya Laboratoreis
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Kikkawa Rie
Pfizer Global Research & Development Toxicology Nagoya Laboratoreis
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Horii Ikuo
Worldwide Safety Sciences Pgrd Nagoya Laboratories Pfizer Inc.
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Yamamoto Toshinori
Departments Of Clinical Pharmacy And Pharmacognosy School Of Pharmaceutical Sciences Showa Universit
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Komiyama Masatoshi
Department Of Bioenvironmental Medicine
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Horii Ikuo
Worldwide Safety Sciences
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Horii Ikuo
Worldwide Safety Sciences Pfizer Global Research & Development Nagoya Laboratories Pfizer Inc.
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Hamada Yoshimasa
Worldwide Safety Sciences Global Research & Development Nagoya Laboratories Pfizer Japan Inc.
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Horii Ikuo
Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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Fukushima Tamio
Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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Yamada Hiroshi
Worldwide Safety Sciences Pfizer Global Research And Development Nagoya Laboratories Pfizer Japan In
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Yamamoto Toshinori
Department Of Pharmacology Medical College Of Oita
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Hamada Yoshimasa
Worldwide Safety Sciences Global Research & Development Nagoya Laboratories Pfizer Inc.
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MORI Chisato
Deparment of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University
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