EFFECTS OF CEPHEM ANTIBIOTICS ON RAT LIVER ALDEHYDE DEHYDROGENASES
スポンサーリンク
概要
- 論文の詳細を見る
Effects of cephem antibiotics, which have a tetrazolethiol side chain, on rat liver mitochondrial aldehyde dehydrogenases (ALDH) were investigated in vitro and in vivo. The antibiotics tested were cefmetazole (CMZ), cefamandole (CMD), cefotiam (CTM), cefoperazone (CPZ) and latamoxef (LMOX). The antibiotics inhibited low-K<SUB>m</SUB> ALDH activity by 17-30% at 5 mM in vitro. The degrees of inhibition were in the order: CMZ=CTM=CMD>LMOX>CPZ. Disulfiram inhibited the enzyme activity by 50% at approx. 40, μM. The antibiotics (except CTM) at a dose of 1, 000 mg/kg i.v. inhibited the low-K<SUB>m</SUB> ALDH activity by 36-52% of the control 24 hr after pretreatment, but did not alter the high-K<SUB>m</SUB> ALDH activity. The degrees of inhibition were in the order: LMOX=CMD>CPZ>CMZ. Disulfiram at a dose of 300 mg/kg p.o. markedly inhibited the low-K<SUB>m</SUB> ALDH activity, but did not alter the high-K<SUB>m</SUB> ALDH activity. The blood acetaldehyde levels during ethanol metabolism were elevated 1.3-2.6 times in rats treated with the cephem antibiotics (except CTM) for 24 hr at a dose of 1, 000 mg/kg i.v. The degrees of elevation at 1 hr after ethanol injection were in the order: LMOX>CMD>CPZ>CMZ. The present experiments demonstrated that the rise in blood acetaldehyde levels coincided with the inhibition rates of the low-K<SUB>m</SUB> ALDH activity by the cephem antibiotics.
- 社団法人 日本薬理学会の論文
著者
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Egashira Toru
Department Of Cardiology Keio University School Of Medicine
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Yamamoto T
Showa Univ. Tokyo Jpn
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Yamamoto Toshinori
Department Of Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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EGASHIRA TORU
Medical College of Oita
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YAMANAKA YASUMITSU
Medical College of Oita
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Yamanaka Yasumitsu
Department Of Pharmacology Oita Medical University
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Egashira Toru
Dept. Pharmacol. Med. College Of Oita
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Yamanaka Y
Department Of Pharmacology Oita Medical University
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Egashira T
Oita Medical Univ. Oita Jpn
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