Ribosomal protein L3 mediated the transport of digoxin in Xenopus laevis oocyte
スポンサーリンク
概要
- 論文の詳細を見る
Ribosomal protein L3 (RPL3) is known to be an indispensable and essential component for the peptidyltransferase center. In the present study, we found a novel function of RPL3 using a Xenopus laevis oocyte expression system. When expressed in X. oocytes, RPL3 mediated the high affinity transport of [3H]digoxin (Km = 213.3 ± 46.8 nM) in a time-, concentration-, and sodium-dependent manners. The maximum velocity of the transport of [3H]digoxin via RPL3 produced at physiological pH. However, we did not observe RPL3-mediated transport of several organic solutes such as [14C]androstenedione, [3H]dexamethasone, [3H]dehydroepiandrosterone sulfate, [3H]L-tryptophan, [14C]L-ascorbic acid, [14C]α-ketoglutarate, [14C]glutarate, [3H]methotrexate, [3H]bumetanide, [3H]probenecid, [14C]salicylic acid, [14C]theophylline and [3H]valproate. Our results suggest that RPL3 functions as a drug carrier protein and may be involved in the digoxin toxicity in the human body.
- 2010-12-01
著者
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Yamamoto Toshinori
Drug Safety Research & Development Pfizer Global Research & Development Nagoya Laboratories
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Yamamoto T
Showa Univ. Tokyo Jpn
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Yamamoto Toshinori
Department Of Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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KOBAYASHI Yasuna
Department of Clinical Pharmacy, Showa University School of Pharmaceutical Sciences
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Yamamoto Toshinori
Departments Of Clinical Pharmacy And Pharmacognosy School Of Pharmaceutical Sciences Showa Universit
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Kohyama Noriko
Department Of Clinical Pharmacy School Of Pharmacy Showa University
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Kohyama Noriko
Department Of Biology Faculty Of Science Ochanomizu University
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KAWAKAMI Kanako
Department of Clinical Pharmacy, School of Pharmacy, Showa University
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OHBAYASHI Masayuki
Department of Clinical Pharmacy, School of Pharmacy, Showa University
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Kobayashi Y
Department Of Clinical Pharmacy School Of Pharmacy Showa University
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Kobayashi Yasuna
Department Of Clinical Pharmacy Showa University School Of Pharmaceutical Sciences
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Ohbayashi Masayuki
Department Of Clinical Pharmacy School Of Pharmacy Showa University
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Kawakami Kanako
Department Of Clinical Pharmacy School Of Pharmacy Showa University
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Yamamoto Toshinori
Department Of Pharmacology Medical College Of Oita
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Kobayashi Yasuna
Department of Pharmacotherapy, Division of Clinical Pharmacy, School of Pharmacy, Showa University
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