Some Interrelated Properties of A and B Form Monoamine Oxidase in Monkey Brain Mitochondria
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概要
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The multiplicity of monoamine oxidase (MAO) in monkey brain was studied by comparing the relationship between the selective substrates of MAO and the pH-activity curves obtained using these substrates. When mitochondrial and A-form MAO were used as the enzyme preparations with serotonin (5-HT) and norepinephrine (NE), preferential substrates for A-form MAO, the pH optima were 8.8 and 7.8 with 5-HT and 8.5 and 7.2 with NE. These substrates were also oxidized by B-form MAO after changing the pH of the incubation medium (shift to alkaline); these pH optima were 9.0 and 8.2, respectively. When common substrates of MAO were used (tyramine, octopamine, dopamine and tryptamine), the pH activity curves obtained were all broad and bell-shaped with pH optima for the 3 species of enzyme (mitochondria, A-form and B-form MAO) at 8.0, 7.8, and 8.0 with tyramine; 8.3, 7.5, and 8.5 with octopamine; 7.8, 7.5, and 8.5 with dopamine; and 8.0, 8.3, and 6.9 with tryptamine, respectively. The pH optima were 6.6 with β-phenylethylamine (β-PEA) and 9.0 with benzylamine, preferential substrates for B-form MAO, for either mitochondria or B-form MAO. The K<SUB>m</SUB> values obtained for tryptamine and β-PEA were lower than those for the other substrates of MAO, regardless of the enzyme preparations. The K<SUB>m</SUB> and V<SUB>max</SUB> values of both forms MAO for 5-HT and NE were similar to those of the A-form MAO. The differences in the K<SUB>m</SUB> and V<SUB>max</SUB> values of the A-form MAO and B-form MAO for common substrates were comparable. Tyramine, octopamine and dopamine were substrates for both forms MAO, with only a slight preference for B-form MAO over A-form MAO. However, tryptamine may be deaminated predominantly by A-form MAO.
著者
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Egashira Toru
Department Of Cardiology Keio University School Of Medicine
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Yamamoto Toshinori
Department Of Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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Yamanaka Yasumitsu
Department Of Pharmacology Oita Medical University
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