Application of Human iPS Cell-Technologies to Arrhythmia Researches
スポンサーリンク
概要
- 論文の詳細を見る
The possibility of modeling human diseases has been a potential in regenerative medicine. Disease modeling especially using human induced pluripotent stem (hiPS) cell is highly applicable for the novel approaches to analyze genetic cardiovascular diseases. Although the inherited fatal arrhythmogenic diseases including long QT syndrome (LQTs) have been characterized by their clinical as well as genomic phenotypes, further investigations for elucidation of pathogenesis and development of tailor-maid therapy are highly desired. We generated hiPS cells from healthy volunteers or patients with LQTs by transfecting 4 reprogmming genes such as Oct3/4, Sox2, Klf4, and c-Myc. Stable iPS colonies were screened by immunostaining and RT-PCR of the various stem cell markers, and cardiac differentiation of hiPS cells were made by formation of embryoid bodies. Molecular characterization of hiPS-derived cardiomyocytes was investigated, and action potential was recorded using conventional microelectrode technique. Furthermore, drug sensitivity test was also performed by multi-electrode array systems. HiPS-derived cardiomyocytes of normal volunteers showed the similar characteristics to native cardiomyocytes on expression of cardiac specific-marker genes and electrophysiologic phenotypes. Notably, LQTs model using patient-specific iPS-derived cardiomyocytes showed the characteristic LQTs phenotype, comparing to healthy control, implicating that disease modeling using hiPS cells may play a crucial role for arrhythmia researches including disease mechanisms as well as development of new therapies.
著者
-
Egashira Toru
Department Of Cardiology Keio University School Of Medicine
-
Fukuda Keiichi
Department Of Cardiology Keio University School Of Medicine
-
Yuasa Shinsuke
Department of Cardiology, Keio University School of Medicine, Japan
-
Egashira Toru
Department of Cardiology, Keio University School of Medicine
-
Murata Mitsushige
Department of Laboratory Medicine, Keio University School of Medicine
-
Murata Mitsushige
Department of Cardiology, Keio University
-
Yae Kojiro
Department of Cardiology, Keio University School of Medicine
-
Yuasa Shinsuke
Department of Cardiology, Keio University School of Medicine
-
Yuasa Shinsuke
Department of Cardiology, Keio University
関連論文
- FRS-035 Pathological Angiogenesis in Human Cardiac Valves is Accompanied by Decreased ChM-I and Augmented VEGF-A and MMP Expression(Pathophysiology of Cardiovascular Disease, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-436 Hydrogen Gas Protects Cardiomyocyte Cell Death and Reduces the Infarct Size in the Rat Model of Myocardial Ischemia-Reperfusion Injury(PE073,Myocardial Ischemia-reperfusion (Basic) (IHD),Poster Session (English),The 73rd Annual Scientific Meeting o
- PE-452 Cardiomyocytes Derived from Bimaternal Parthenogenetic ES Cells by Epigenetic Modification have a Different Property and Genetic Presentation in Cardiogenesis(PE076,Regeneration (M),Poster Session (English),The 73rd Annual Scientific Meeting of the
- PE-021 Inadequate Suppression of Aldosterone Biosynthesis after Salt Intake is a Primary Cause of Sodium-Retention in Dahl Salt-Sensitive Rat(PE004,Hypertension, Basic (H),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circula
- OE-168 Atf4 Plays a Key Role in Antioxidant Stress Response in the Heart(OE29,Molecular Biology Myocardium (M),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OE-167 MiR-142-3p Regulates Heart Development and Somitegenesis on the Stage of Early Mesoderm Formation(OE29,Molecular Biology Myocardium (M),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-908 Cardiac-Specific Defense Mechanisms against Lipid Peroxidation Products(Metabolism / Biochemistry / Energetics(03)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-905 Remodeling of Cardiac Energy Metabolism under Oxidative Stress to Enhance Regeneration and de novo Synthesis of Glutathione(Metabolism / Biochemistry / Energetics(03)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Ci
- PJ-274 Wnt2 plays a key role in cardiac development via a non-canonical pathway(Development and differentiation(02)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-225 Inhalation of hydrogen gas protects the heart from ischemic reperfusion injury(Myocardial ischemia/reperfusion, basic/clinical(03)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-337 Activin A can Induce the Differentiation of Cardiomyocytes from Medaka Embryonic Cells in vitro(Development and differentiation(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-240 Cardiomyocytes can be Generated from Parthenogenetic Embryonic Stem (ES) Cells(Regeneration(angiogenesis/myocardial regeneration)(01)(M),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-568 Contribution of Bone Marrow-derived Cells on Pressure Overload-induced Cardiac Hypertrophy and Perivascular Fibrosis in Mice(Regeneration (angiogenesis/myocardial regeneration)-4, The 71st Annual Scientific Meeting of the Japanese Circulation Socie
- OE-255 Bone Marrow-derived Cells are Mobilized and Contribute to Cardiac Hypertrophy via Cell Fusion and Transdifferentiation Mechanism(Regeneration (angionenesis/myocardial regeneration)-1 (M) OE43,Oral Presentation (English),The 70th Anniversary Annual
- Cardiac Side Population-derived Cardiosphere from Rats Migrated and Differentiated into Neural Crest-derived Cells in Chicken Embryo(Development and Differentiation 1 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Molecular Characterization and Pluripotency of the Cardiac Side Population Cells(Development and Differentiation 2 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- PE-258 Versican is Essential for Ventricular Trabeculation and Outflow Tract Formation in Medaka Heart(PE044,Molecular Biology (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OE-138 Neural Crest Stem Cells Supply Intrinsic Cardiac Adrenergic Cells and Contribute to Reinnervation after Myocardial Infarction in Mice(OE24,Autonomic Nervous System (H),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese C
- FRS-002 Yes-associated Protein (YAP) is Essential for the Linear Heart Tube Formation in Cardiac Development(FRS1,New Molecules for Cardiovascular Regulation (M),Featured Research Session (English),The 73rd Annual Scientific Meeting of The Japanese Circul
- 2 Metabolome analysis revealed the inherent capacity of heart to acquire enhanced tolerance to oxidative stress via metabolic reprogramming(Symposium 7 (SY-07) (M) Molecular Mechanism and Novel Therapeutic Target in Heart Failure,Special Program,The 72nd
- OE-251 Molecular Characterization, Safety and Feasibility of Induced Pluripotent Stem (iPS) Cell-derived Cardiomyocytes for Heart Regenerative Therapy(OE43,Regeneration (M),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Cir
- OE-241 "Re-aggregation Method" Dramatically Improved the Survival of Purified Mouse and Monkey ES Cell-Derived Cardiomyocytes in Grafted Myocardium(Regeneration(angiogenesis/myocardial regeneration)(01)(M),Oral Presentation(English),The 72nd Annual Scient
- 1 Cardiomyocyte Differentiation from Embryonic Stem Cells(Regenerative Medicine of the Cardiovascular Field,Japan-Korea Cardiovascular Conference,Presidential Planning Program (M),Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulat
- FRS-056 Heat-shock Protein 60 is Required for Cardiac Regeneration in Zebrafish(Regeneration, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-769 Glucocorticoid Protects Heart from Ischemia-reperfusion Injury through Activation of Lipocalin-type Prostaglandin D Synthasederived PGD2 Biosynthesis(PJ129,Myocardial Ischemia-reperfusion (Basic, Clinical) (IHD),Poster Session (Japanese),The 73rd A
- PJ-187 Adeno-associated Virus-mediated Prostaglandin I2 Synthase (PGIS) Gene Transfer Improves a Limb Ischemia(PJ032,Peripheral Circulation/Vascular Disease (Pathophysiology, Basic) 1 (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The
- PE-358 The Potent Angiogenic Factor Periostin Accelerates Degeneration and Sclerosis of the Cardiac Valve Complex(PE060,Valvular Heart Disease (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PE-262 Kinase-dependent Metabolic Shift towards Phgdh-mediated Serine Synthesis Enhances Cardioprotection to Oxidative Stress as a Hormetic Response to Aldehydes(PE044,Molecular Biology (M),Poster Session (English),The 73rd Annual Scientific Meeting of Th
- PE-261 Wnt2 Plays a Key Role in Cardiac Development via a Non-canonical Pathway(PE044,Molecular Biology (M),Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OE-251 Molecular Characterization, Safety and Feasibility of Induced Pluripotent Stem (iPS) Cell-derived Cardiomyocytes for Heart Regenerative Therapy(OE43,Regeneration (M),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japanese Cir
- 1 Local Tenomodulin Absence, Angiogenesis, and Matrix Metalloproteinase Activation are Associated with the Rupture of the Chordae Tendineae Cordis(Young Investigator's Award Finalists Lectures (YIA),The 73rd Annual Scientific Meeting of The Japanese Circu
- OE-325 Myocardial Cell Sheet (MCS) Transplantation Combined with Omentopexy Synergistically Improved Cardiac Function and Remodeling after Myocardial Infarction(Regeneration (angionenesis/myocardial regeneration)-2 (M) OE55,Oral Presentation (English),The
- 2 Heart Failure Causes Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via Gp130-mediated Cytokines(Young Investigator's Award Finalists Lectures (YIA),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-172 AAV-PGIS Gene Transfer Improves Hypoxia-induced Pulmonary Hypertension in Mice(Pulmonary circulation(03)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-017 Characterization of the Rejuvenation of Cardiac Sympathetic Nerves in Cardiac Hypertrophy(Autonomic nervous system(02)(H),Oral Presentation(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-315 Acute Cardiac Hypertrophy Causes Sympathetic Nerve Dysfunction by Inducing Immature and Fetal Gene Expression(Cardiac hypertrophy, basic/clinical-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-226 Nerve Growth Factor is Critical for Cardiac Sensory Nerve Development(Autonomic nervous system-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-225 Nerve Growth Factor Rescues Cardiac Sensory Innervation and Silent Myocardial Ischemia in Diabetic Hearts(Autonomic nervous system-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-571 Neural Crest Stem Cells Contribute to Adipose Tissue-derived Tissue Stem Cells and Can Differentiate into Cardiomyocytes(Regeneration (angiogenesis/myocardial regeneration)-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-O98 Sema3A Produces Cardiac Ventricular Repolarization Gradient via Sympathetic Innervation Patterning(Autonomic nervous system-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-O95 Cardiac Hypertrophy induces Sympathetic Hyperinnervation concomitant with the Elevation of Endothelin-1 and Nerve Growth Factor(Autonomic nervous system-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-058 Neural Crest-Derived Stem Cell in the Heart Proliferate and Differentiate into Cardiomyocytes after Myocardial Infarction(Regeneration, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-301 Cardiac Neural Crest-Derived Cells Lay Hidden as Dormant Stem Cells and Differentiate into Cardiomyocytes in Vivo(Development and differentiation-1 (M) OE51,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese
- Cardiac Regenerative Medicine(Treatment)
- Dominant negative suppression of Rad leads to QT prolongation and causes ventricular arrhythmias via modulation of L-type Ca^ channels in the heart
- -0196-EVALUATION OF TRICUSPID REGURGITATION BY VENA CAVAL FLOW PATTERN USING A CATHETER-TIP DOPPLER VELOCIMETER
- PJ-402 Disruption of Chondromodulin-I Gene, a Potent Angioinhibitory Factor, Causes Aortic Valve Stenosis in Mice(Valvular herat disease/Pericarditis/Cardiac tumor-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-222 Evidence that Advanced Parkinson's Disease reveals Complete Cardiac Sympathetic Denervation leading to Cardiovascular Collapse(Autonomic nervous system-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-592 Periostin is a Potent Anglogenic Factor and Involved in Degeneration of the Cardiac Valves(Valvular heart disease/Pericarditis/Cardiac tumor(02)(M),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- Determination of the autocrine/paracrine secreted cytokines and growth factors in bone marrow derived cardiomyogenic cells
- Molecular mechanism of the changes in appearance of the action potentials in bone-marrow derived regenerated cardiomyocytes
- 2 Regulation of Sarcoplasmic Reticulum Ca2+ Leak by Small G-protein Rad in the Heart(Subcellular Remodeling in the Failing Heart,Symposium 10 (SY-10) (A),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- 4 Deregulation of Small G-protein Rad Affects L-type Calcium Channel Function and Ca^ Homeostasis by α Subunit-trafficking in Heart Failure(Plenary Session 5 (PL-5) (M) Calcium Handling Abnormality in Heart Failure,Special Program,The 72nd Annual Scie
- OE-083 Dominant Negative Suppression of Rad Prolongs QT Interval and Causes Ventricular Arrhythmias in Mice(Arrhythmia, basic-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OE-175 Nerve Growth Factor is Critical for Cardiac Sensory Innervation and Rescues Neuropathy in Diabetic Hearts(Neurohumoral factors-1 (H) OE30,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society
- 2 Zac1 is an essential transcription factor in cardiac morphogenesis(Young Investigator's Award Finalists Lectures (YIA),Special Program,The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- PE-313 An Angioinhibitory Factor of Chondrocyte (Chondromodulin-i) Plays a Critical Role in Maintaining Cardiac Valvular Function(Vascular heart disease/Pericarditis/Cardiac tumor-2 (M) PE53,Poster Session (English),The 70th Anniversary Annual Scientific
- COMPARISON OF THE EFFECT OF AN EQUIMOLAR AND LOW DOSE OF FENITROTHION AND METHYLPARATHION ON THEIR OWN METABOLISM IN RAT LIVER
- PROTECTIVE EFFECTS OF DIISOPROPYL 1, 3-DITHIOL-2-YLIDENE MALONATE (NKK-105) ON LIVER INJURY BY CARBON TETRACHLORIDE (CCl_4)
- Protective Effect of Ninjin-yoei-to on Damage to Isolated Hepatocytes Following Transient Exposure to tert-Butly Hydroperoxide
- Day/Night Variation of 5-Hydroxyindole Acetic Acid Concentration in Rat Cerebrospinal Fluid After Acute and Long-Term Administration of a Selective Serotonin Reuptake Inhibitor, Fluvoxamine
- The Inhibition of Monoamine Oxidase Activity by Various Antidepressants: Differences Found in Various Mammalian Species
- Monitoring of Radical Scavenging Activity of Peroral Administration of the Kampo Medicine Sho-saiko-to in Rats
- Variation of Monoamines and Their Metabolite Contents in the Cerebrospinal Fluid of Conscious Rats
- "P-17 NO contribution to lipopolysaccharide-induced hepatic damage of galactosamine-sensitized mice.
- Effects of Bifemelane on Muscarinic Receptors and Choline Acetyltransferase in the Brains of Aged Rats Following Chronic Cerebral Hypoperfusion Induced by Permanent Occlusion of Bilateral Carotid Arteries
- Effect of Fluvoxamine on 5-Hydroxytryptamine Uptake, Paroxetine Binding Sites and Ketanserin Binding Sites in the Japanese Monkey Brain and Platelets, In Vivo and In Vitro
- Role of oxygen-derived free radicals and lipid peroxidation in blood induced by lipopolysaccharide(LPS).
- THE MULTIPLE HYDROPEROXIDES OF CHOLINE PHOSPHOLIPIDS OCCURRING IN PLASMA AFTER ISCHEMIA-REPERFUSION IN RAT LIVER
- Idenitification of phospholipid hydroperoxides in plasma of hepatic ischemiareperfused rats
- W-4 Investigation of the Other Organ Injury Caused by Liver Ischemia-Reperfusion
- MONOAMINE OXIDASE (MAO) IN PLASMA OF RATS AFTER HEPATOTOXINS ADMINISTRATION
- Antioxidant and Hepatoprotective Actions of the Medicinal Herb Artemisia campestris from the Okinawa Islands
- Inhibition by Zn^ of A-form Monoamine Oxidase in Monkey Brain Mitochondria
- Effect of Long-Term Fluvoxamine Treatment on Endogenous Serotonin Uptake Inhibitor-Like Substance in Monkey Cerebrospinal Fluid
- METHAMPHETAMINE-INDUCED BEHAVIORAL EFFECTS AND RELEASES OF BRAIN CATECHOLAMINES AND BRAIN CONCENTRATIONS OF METHAMPHETAMINE IN MICE
- EFFECTS OF CEPHEM ANTIBIOTICS ON RAT LIVER ALDEHYDE DEHYDROGENASES
- NADPH-DEPENDENT LIPID PEROXIDATION IN THE LIVER MICROSOME OF RATS FED A 3-METHYL-4-DIMETHYL-AMINOAZOBENZENE (3-Me-DAB)-CONTAINING DIET
- Effect of Methylprednisolone on Plasma Lipid Peroxidation Induced by Lipopolysaccharide
- SUPPRESSIVE EFFECT OF BIFEMELANE ON LIPID PEROXIDATION IN RAT LIVER
- NO CONTRIBUTION TO LIPOPOLYSACCHARIDE-INDUCED HEPATIC DAMAGE IN GALACTOSAMINE-SENSITIZED MICE
- EFFECTS OF ETHANOL ON CATECHOLAMINE LEVELS AND RELATED ENZYME ACTIVITIES IN DIFFERENT BRAIN REGIONS OF RATS
- FURTHER STUDIES ON THE SYNTHESIS OF A-FORM MONOAMINE OXIDASE
- INHIBITION OF MONOAMINE OXIDASE BY 3-METHYL-4-DIMETHYLAMINO AZOBENZENE (3-Me-DAB) IN RAT LIVER MITOCHONDRIA
- EFFECT OF SODIUM NITRITE ON MONOAMINE OXIDASE ACTIVITY IN RAT LIVER AND BRAIN
- SYNTHETIC RATES OF MONOAMINE OXIDASE IN RAT LIVER AFTER CLORGYLINE OR DEPRENYL ADMINISTRATION
- B-8 An injury of the liver caused by ischemia-reperfusion in rat liver.
- Attenuation of Dysfunction in the Ischemia-Reperfused Liver by Glycyrrhizin
- Characteristics of Mitochondrial and Synaptosomal Monoamine Oxidase in Monkey Brain
- Some Interrelated Properties of A and B Form Monoamine Oxidase in Monkey Brain Mitochondria
- PRODUCTION OF TOLERANCE TO FENITROTHION I N MALE RATS
- Three-dimensional echocardiography findings of biventricular thrombi complicated by cerebral embolism
- Application of Human iPS Cell-Technologies to Arrhythmia Researches
- Novel Insights into Disease Modeling Using Induced Pluripotent Stem Cells
- Effects of d-methamphetamine on monkey brain monoamine oxidase, in vivo and in vitro.
- 18F-FDG-PET CT Findings in a Patient with Early-phase Cardiac Sarcoidosis
- Changes in amine oxidase in plasma of rats treated with hepatotoxins.
- Effect of bifemelane hydrochloride on an injury of the liver caused by ischemia-reperfusion in rats.
- Changes in MAO activities in several organs of rats after administration of l-thyroxine.
- Studies on monoamine oxidase. XVIII. Enzymic properties of placental monoamine oxidase.