EFFECTS OF PLURONIC F-127 ON LOADING OF FURA 2/AM INTO SINGLE SMOOTH MUSCLE CELLS ISOLATED FROM GUINEA PIG TAENIA COLI
スポンサーリンク
概要
- 論文の詳細を見る
The effect of Pluronic F-127 (PF-127), a surfactant polyol, on the loading of fura 2/AM into smooth muscle cells isolated from guinea pig taenia coli was investigated. The presence of PF-127 during the loading of fura 2 acetoxymethyl ester (fura 2/AM), an intracelluler Ca indicator, occasionally increased the incorporation of the dye into the cells. However, long time loading (over approximately 60 min) in the presence of PF-127 decreased the incorporation of fura 2. When the extracellular medium obtained from cells treated with DFP, then incubated with fura 2/AM in the presence of PF-127 was analyzed with excitation spectra, the fluorescence peaks shifted to longer wave lengths by addition of EGTA. However, the peak of the ektracellular medium obtained from cells treated with DFP, then incubated without PF-127, did not shift. These results show that PF-127 affects the membranous permeability of the dye in single smooth muscle cells, permititing fura 2 hydrolyzed in the cytoplasm to leak out through the membrane.
- 日本トキシコロジー学会の論文
- 1989-08-25
著者
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MOMOSE Kazutaka
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
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Yamamoto Toshinori
Department Of Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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Maruyama Ikuo
Department Of Laboratory And Molecular Medicine Kagoshima University School Of Medicine
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Momose K
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
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Momose Kazutaka
Department Of Phamacology School Of Pharmaceutical Sciences Showa University
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HASEGAWA Tomohiko
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
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Hasegawa T
Department Of Pharmacology School Of Pharmaceutical Sciences Showa University
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MARUYAMA Ikuo
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University
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